CAJ | 학술논문

建立高效液相色谱法同时测定白花蛇舌草药材中对香豆酸和熊果酸含量的方法。采用 Eclipse SB–C18色谱柱(250 mm×4.6 mm，5μm)，以甲醇–0.05%甲酸梯度洗脱，流量1 mL／min，对香豆酸和熊果酸的检测波长分别为310 nm 和210 nm，柱温30℃。对香豆酸和熊果酸含量分别在0.00179～0.02864 mg／mL 和0.0213～0.3408 mg／mL范围内与色谱峰面积呈良好的线性关系，线性相关系数 r 分别为0.9998，0.9996。对香豆酸和熊果酸的平均加标回收率分别为98.59%，98.23%，测定结果的相对标准偏差分别为0.81%，0.94%(n=6)。样品溶液在24 h 内稳定。该方法适用于白花蛇舌草药材中对香豆酸和熊果酸的含量测定。
건립고효액상색보법동시측정백화사설초약재중대향두산화웅과산함량적방법。채용 Eclipse SB–C18색보주(250 mm×4.6 mm，5μm)，이갑순–0.05%갑산제도세탈，류량1 mL／min，대향두산화웅과산적검측파장분별위310 nm 화210 nm，주온30℃。대향두산화웅과산함량분별재0.00179～0.02864 mg／mL 화0.0213～0.3408 mg／mL범위내여색보봉면적정량호적선성관계，선성상관계수 r 분별위0.9998，0.9996。대향두산화웅과산적평균가표회수솔분별위98.59%，98.23%，측정결과적상대표준편차분별위0.81%，0.94%(n=6)。양품용액재24 h 내은정。해방법괄용우백화사설초약재중대향두산화웅과산적함량측정。
A method for simultaneously determination of p-coumaric acid and ursolic acid in hedyotis diffusa was established. An Eclipse SB–C18 column (250 mm×4.6 mm,5 μm) was adopted with a mobile phase of methanol–0.05%formic acid at the flow rate of 1.0 mL/min. The detection wavelength was set at 310 nm and 210 nm for p-coumaric acid, ursolic acid, respectively, and the column temperature was 30oC. The calibration curves were linear in the ranges of 0.001 79–0.028 64 mg/mL(r=0.999 8) for p-coumaric acid and 0.021 3–0.340 8 mg/mL(r=0.999 6) for ursolic acid. The average recoveries of p-coumaric acid and ursolic acid were 98.59% and 98.23%,respectively,and the detection limit of p-coumaric and ursolic acid was 0.81%,0.94%(n=6),respectively. The sample solution was steady within 24 h. The method is suitable for determination of p-coumaric acid and ursolic acid in hedyotis diffusa.