中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2014年
12期
881-886
,共6页
三氯乙烯%变态反应%细胞免疫%体液免疫
三氯乙烯%變態反應%細胞免疫%體液免疫
삼록을희%변태반응%세포면역%체액면역
Trichloroethylene%allergy%Cellular immunity%Humoral immunity
目的 探讨三氯乙烯(TCE)致变态反应是否存在细胞免疫和体液免疫共同参与,为研究其发病机制提供科学依据.方法 应用豚鼠和大鼠进行实验,分别设立阴性对照组、阳性对照组、TCE实验组,用皮内注射方式分别注射橄榄油、2,4-二硝基氯苯(DNCB)和TCE.实验结束后收集大鼠外周血液,用流式细胞仪检测淋巴细胞CD3+、CD4+、CD8+比例;收集豚鼠外周血液测定IgG、IgA、IgM、C3、C4水平;收集豚鼠脾淋巴细胞,用荧光定量PCR检测免疫相关基因GATA3、T-bet、CTLA4和Foxp3的mRNA表达水平.此外,选取TCE药疹样皮炎患者作为病例组,采用荧光定量PCR检测外周血Foxp3、GATA3、CTLA4、T-bet的mRNA表达水平.结果 (1)TCE对豚鼠皮肤有明显致敏作用,致敏率为83.3％;TCE实验组和阳性对照组IgG水平比阴性对照组显著升高(P＜0.01);TCE实验组和阳性对照组GATA3、T-bet、CTLA4 mRNA表达水平显著高于阴性对照组,Foxp3 mRNA表达水平低于阴性对照组.(2)TCE实验组和阳性对照组大鼠外周血淋巴细胞CD3+比例高于阴性对照组,TCE实验组CD4+、CD8+、CD4+/CD8+与阴性对照组比较无统计学差异.(3)TCE病例组Foxp3、GATA3、CTLA4 mRNA表达水平比对照组分别升高115％、97％和241％(P＜0.01),T-bet mRNA表达水平下降47％(P＜0.01).结论 TCE可引起细胞免疫和体液免疫发生明显改变,说明TCE导致的免疫损伤属于细胞免疫和体液免疫共同参与的混合型变态反应,可能是Ⅳ型和Ⅱ型变态反应.
目的 探討三氯乙烯(TCE)緻變態反應是否存在細胞免疫和體液免疫共同參與,為研究其髮病機製提供科學依據.方法 應用豚鼠和大鼠進行實驗,分彆設立陰性對照組、暘性對照組、TCE實驗組,用皮內註射方式分彆註射橄欖油、2,4-二硝基氯苯(DNCB)和TCE.實驗結束後收集大鼠外週血液,用流式細胞儀檢測淋巴細胞CD3+、CD4+、CD8+比例;收集豚鼠外週血液測定IgG、IgA、IgM、C3、C4水平;收集豚鼠脾淋巴細胞,用熒光定量PCR檢測免疫相關基因GATA3、T-bet、CTLA4和Foxp3的mRNA錶達水平.此外,選取TCE藥疹樣皮炎患者作為病例組,採用熒光定量PCR檢測外週血Foxp3、GATA3、CTLA4、T-bet的mRNA錶達水平.結果 (1)TCE對豚鼠皮膚有明顯緻敏作用,緻敏率為83.3％;TCE實驗組和暘性對照組IgG水平比陰性對照組顯著升高(P＜0.01);TCE實驗組和暘性對照組GATA3、T-bet、CTLA4 mRNA錶達水平顯著高于陰性對照組,Foxp3 mRNA錶達水平低于陰性對照組.(2)TCE實驗組和暘性對照組大鼠外週血淋巴細胞CD3+比例高于陰性對照組,TCE實驗組CD4+、CD8+、CD4+/CD8+與陰性對照組比較無統計學差異.(3)TCE病例組Foxp3、GATA3、CTLA4 mRNA錶達水平比對照組分彆升高115％、97％和241％(P＜0.01),T-bet mRNA錶達水平下降47％(P＜0.01).結論 TCE可引起細胞免疫和體液免疫髮生明顯改變,說明TCE導緻的免疫損傷屬于細胞免疫和體液免疫共同參與的混閤型變態反應,可能是Ⅳ型和Ⅱ型變態反應.
목적 탐토삼록을희(TCE)치변태반응시부존재세포면역화체액면역공동삼여,위연구기발병궤제제공과학의거.방법 응용돈서화대서진행실험,분별설립음성대조조、양성대조조、TCE실험조,용피내주사방식분별주사감람유、2,4-이초기록분(DNCB)화TCE.실험결속후수집대서외주혈액,용류식세포의검측림파세포CD3+、CD4+、CD8+비례;수집돈서외주혈액측정IgG、IgA、IgM、C3、C4수평;수집돈서비림파세포,용형광정량PCR검측면역상관기인GATA3、T-bet、CTLA4화Foxp3적mRNA표체수평.차외,선취TCE약진양피염환자작위병례조,채용형광정량PCR검측외주혈Foxp3、GATA3、CTLA4、T-bet적mRNA표체수평.결과 (1)TCE대돈서피부유명현치민작용,치민솔위83.3％;TCE실험조화양성대조조IgG수평비음성대조조현저승고(P＜0.01);TCE실험조화양성대조조GATA3、T-bet、CTLA4 mRNA표체수평현저고우음성대조조,Foxp3 mRNA표체수평저우음성대조조.(2)TCE실험조화양성대조조대서외주혈림파세포CD3+비례고우음성대조조,TCE실험조CD4+、CD8+、CD4+/CD8+여음성대조조비교무통계학차이.(3)TCE병례조Foxp3、GATA3、CTLA4 mRNA표체수평비대조조분별승고115％、97％화241％(P＜0.01),T-bet mRNA표체수평하강47％(P＜0.01).결론 TCE가인기세포면역화체액면역발생명현개변,설명TCE도치적면역손상속우세포면역화체액면역공동삼여적혼합형변태반응,가능시Ⅳ형화Ⅱ형변태반응.
Objective To investigate whether cellular immunity and humoral immunity are involved in trichlorethylene (TCE)-induced mixed allergy,then provide the scientific basis for the mechanism of this disease.Methods Guinea pigs and rats were tested for this study by application of guinea pig maximization test (GPMT),the animals were randomly divided into negative control,positive control and TCE treatment groups.Animals of these groups were administrated with olive oil,2,4-dinitrochlorobenzene (DNCB),and TCE,respectively,by intradermal injection.After TCE administration,rat peripheral blood samples were collected by flow cytometry to detect lymphocytes CD3+,CD4+,CD8+.Guinea pig peripheral blood samples were collected to detect the levels of IgG,IgA,IgM,C3,C4,and the spleens were taken out from guinea pigs after various treatment,mRNA expression of GATA3,T-bet,CTLA4 and Foxp3 in lymphocytes of guinea pig spleen was detected by real-time fluorescent PCR assay.Additionally,TCE allergic dermatitis patients were selected for the study,the peripheral blood samples were collected from the TCE patients group and control group,quantitative PCR was applied to detect mRNA expression of immune-related genes Foxp3,GATA3,CTLA4,T-bet.Results TCE induced obvious skin allergic reaction in guinea pigs,the sensitization rate was 83.3％,IgG levels in TCE group and positive control increased significantly.Additionally,mRNA expression levels of GATA3,T-bet,CTLA4 significantly elevated in TCE group and positive control,but Foxp3 mRNA levels decreased.The lymphocytes CD3+ ratio in TCE group and positive control of rats was higher than that in negative control,we found that there was no statistical difference of CD4+,CD8+,CD4+/CD8+ between TCE group and negative control of rats.The mRNA expression levels of Foxp3,GATA3,CTLA4 in TCE patients increased by 115％,97％,241％,respectively as compared with the control,T-bet levels decreased by 47％ when compared with the control.Conclusions TCE could induce obvious changes of cellular immunity and humoral immunity in guinea pigs,rats,and TCE patients,these findings indicated that TCE-induced immunological disorder belongs to the mixed allergy with involvment of cellular immunity and humoral immunity,the mixed allergy might be type Ⅳ and type Ⅱ allergy.
