中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2014年
12期
936-940
,共5页
伊雪%邬鹏宇%杨学慧%王耕野%王耕银%崔翔宇%王帅%杨方%李占清
伊雪%鄔鵬宇%楊學慧%王耕野%王耕銀%崔翔宇%王帥%楊方%李佔清
이설%오붕우%양학혜%왕경야%왕경은%최상우%왕수%양방%리점청
肾移植%褪黑素%细胞凋亡%缺血再灌注损伤%小管损伤指数
腎移植%褪黑素%細胞凋亡%缺血再灌註損傷%小管損傷指數
신이식%퇴흑소%세포조망%결혈재관주손상%소관손상지수
Kidney transplantation%Melatonin%Apoptosis%Ischemia reperfusion injury%Tubular damage index
目的 探讨褪黑素预处理受体对移植肾的保护作用及其可能的机制. 方法 40只Lewis大鼠应用随机数字表随机分为对照组和实验组,每组20只(供、受体各10只).实验组:50 mg/kg褪黑素+5 ml牛奶在移植前2h经胃管注入受体大鼠;对照组:5 ml牛奶在移植前2h经胃管注入受体大鼠.移植后0、6和24 h分别采血检测血尿素氮(BUN)和肌酐(Cr)及天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、乳酸脱氢酶(lactate dehydrogenase,LDH);移植后24 h取移植肾检测肾组织中超氧化物歧化酶(superoxide dismutase,SOD)及脂质过氧化物(lipid hydroperoxide,LPO);过碘酸-雪夫染色(PAS染色)观察组织学变化,免疫组化法检测肾移植物中核转录因子-κB (nuclear factor kappa B,NF-κB)和半胱氨酸蛋白酶-3(caspase-3)阳性肾小管数. 结果 实验组与对照组移植后6 h BUN分别为(36.26± 19.05)、(53.64± 16.57) mmol/L,移植后24 h血Cr分别为(144.09±76.91)、(259.01±132.60) μmol/L,组间比较差异均有统计学意义(P＜0.05).移植后24 h,实验组血清AST、ALT、LDH均低于对照组,组间比较差异均有统计学意义(P＜0.05).移植后24 h实验组和对照组移植肾组织中SOD分别为(8.91±4.38)、(2.21 ±0.45) U/mg,LPO分别为(9.5±5.8)、(60.8±23.7) pmol/mg,组间比较差异均有统计学意义(P＜0.05).两组肾小管损伤指数分别为1.50±0.22、3.00±0.15,NF-κB阳性小管评分为2.00±0.21、3.60±0.16,caspase-3阳性小管评分为2.77±0.48、3.38±0.52,组间比较差异均有统计学意义(P＜0.05). 结论 褪黑素预处理受体可能通过降低体内的氧化应激反应,提高抗氧化酶活性,增强清除自由基的能力,抑制脂质过氧化反应和凋亡的发生来减轻移植肾的缺血再灌注损伤,提高移植肾功能.
目的 探討褪黑素預處理受體對移植腎的保護作用及其可能的機製. 方法 40隻Lewis大鼠應用隨機數字錶隨機分為對照組和實驗組,每組20隻(供、受體各10隻).實驗組:50 mg/kg褪黑素+5 ml牛奶在移植前2h經胃管註入受體大鼠;對照組:5 ml牛奶在移植前2h經胃管註入受體大鼠.移植後0、6和24 h分彆採血檢測血尿素氮(BUN)和肌酐(Cr)及天鼕氨痠轉氨酶(AST)、丙氨痠轉氨酶(ALT)、乳痠脫氫酶(lactate dehydrogenase,LDH);移植後24 h取移植腎檢測腎組織中超氧化物歧化酶(superoxide dismutase,SOD)及脂質過氧化物(lipid hydroperoxide,LPO);過碘痠-雪伕染色(PAS染色)觀察組織學變化,免疫組化法檢測腎移植物中覈轉錄因子-κB (nuclear factor kappa B,NF-κB)和半胱氨痠蛋白酶-3(caspase-3)暘性腎小管數. 結果 實驗組與對照組移植後6 h BUN分彆為(36.26± 19.05)、(53.64± 16.57) mmol/L,移植後24 h血Cr分彆為(144.09±76.91)、(259.01±132.60) μmol/L,組間比較差異均有統計學意義(P＜0.05).移植後24 h,實驗組血清AST、ALT、LDH均低于對照組,組間比較差異均有統計學意義(P＜0.05).移植後24 h實驗組和對照組移植腎組織中SOD分彆為(8.91±4.38)、(2.21 ±0.45) U/mg,LPO分彆為(9.5±5.8)、(60.8±23.7) pmol/mg,組間比較差異均有統計學意義(P＜0.05).兩組腎小管損傷指數分彆為1.50±0.22、3.00±0.15,NF-κB暘性小管評分為2.00±0.21、3.60±0.16,caspase-3暘性小管評分為2.77±0.48、3.38±0.52,組間比較差異均有統計學意義(P＜0.05). 結論 褪黑素預處理受體可能通過降低體內的氧化應激反應,提高抗氧化酶活性,增彊清除自由基的能力,抑製脂質過氧化反應和凋亡的髮生來減輕移植腎的缺血再灌註損傷,提高移植腎功能.
목적 탐토퇴흑소예처리수체대이식신적보호작용급기가능적궤제. 방법 40지Lewis대서응용수궤수자표수궤분위대조조화실험조,매조20지(공、수체각10지).실험조:50 mg/kg퇴흑소+5 ml우내재이식전2h경위관주입수체대서;대조조:5 ml우내재이식전2h경위관주입수체대서.이식후0、6화24 h분별채혈검측혈뇨소담(BUN)화기항(Cr)급천동안산전안매(AST)、병안산전안매(ALT)、유산탈경매(lactate dehydrogenase,LDH);이식후24 h취이식신검측신조직중초양화물기화매(superoxide dismutase,SOD)급지질과양화물(lipid hydroperoxide,LPO);과전산-설부염색(PAS염색)관찰조직학변화,면역조화법검측신이식물중핵전록인자-κB (nuclear factor kappa B,NF-κB)화반광안산단백매-3(caspase-3)양성신소관수. 결과 실험조여대조조이식후6 h BUN분별위(36.26± 19.05)、(53.64± 16.57) mmol/L,이식후24 h혈Cr분별위(144.09±76.91)、(259.01±132.60) μmol/L,조간비교차이균유통계학의의(P＜0.05).이식후24 h,실험조혈청AST、ALT、LDH균저우대조조,조간비교차이균유통계학의의(P＜0.05).이식후24 h실험조화대조조이식신조직중SOD분별위(8.91±4.38)、(2.21 ±0.45) U/mg,LPO분별위(9.5±5.8)、(60.8±23.7) pmol/mg,조간비교차이균유통계학의의(P＜0.05).량조신소관손상지수분별위1.50±0.22、3.00±0.15,NF-κB양성소관평분위2.00±0.21、3.60±0.16,caspase-3양성소관평분위2.77±0.48、3.38±0.52,조간비교차이균유통계학의의(P＜0.05). 결론 퇴흑소예처리수체가능통과강저체내적양화응격반응,제고항양화매활성,증강청제자유기적능력,억제지질과양화반응화조망적발생래감경이식신적결혈재관주손상,제고이식신공능.
Objective To investigate the protection and mechanism of melatonin against the adverse effects of ischemia reperfusion injury (IRI) in kidney graft tissue.Methods Forty Lewis rats were randomly divided into 2 groups (donor and recipient individually 10 animals).Melatonin (50 mg/kg.bw) and 5 ml milk were given by gavage for recipient 2 h before kidney transplantation in experiment group.Controls were given the same volume of milk only.Subsequently,left kidney grafts stored in 4 ℃ Histidine-Tryptophan-Ketoglutarate solution for 24 h were transplanted into bilaterally nephrectomized syngeneic recipients.Blood was detected 0,6,and 24 h after reperfusion from recipient's eye vein or vena cava for analysis of serum blood urea nitrogen (BUN),creatinine,transaminases,and lactate dehydrogenase (LDH).Biopsies were taken 24 h after reperfusion to evaluate tubular damage,the enzymatic activity of superoxide dismutase (SOD),lipid hydroperoxide (LPO),nuclear factor kappa B (NF-κB),caspase-3 as indices of oxidative stress,necrosis and apoptosis,respectively.Results Serum BUN decreased 6 h after reperfusion and serum creatiuine decreased 24 h after transplantation in melatonin group compared with those in controls (36.26±19.05 mmol/L versus 53.64±16.57 mmol/L,144.09t76.91 μmol/L versus 259.01 ± 132.60 μ.mol/L,P＜0.05).Serum aspartate aminotransferase,alanine aminotransferase,and LDH decreased significantly 24 h after reperfusion in melatonin group compared with those in controls (P＜0.05).At the same time,kidney graft tubular damage index,NF-κB and caspase-3 immunohistochemistry expression also decreased than those in controls (1.50±0.22 versus 3.00t0.15,2.00±0.21 versus 3.60±0.16,2.77±0.48 versus 3.38±0.52,P＜0.05).Kidney graft tissue SOD activation increased,while LPO concentration decreased in melatonin group (8.91±4.38 U/mg versus 2.21±0.45 U/mg and 9.5±5.8 pmol/mg versus 60.8±23.7 pmol/mg,P＜0.05).℃onclusions Recipient preconditioning with melatonin might reduces significantly adverse effects of IRI and improves graft function in experimental kidney transplantation,likely through reduce of anti-oxidation,increase of anti-apoptosis,improvement of clean up reactive oxygen species,and inhibition of lipid peroxidation.
