中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2014年
10期
621-625
,共5页
周立杰%王现雷%谭金锋%卢志方%宋春雨%崔晓光
週立傑%王現雷%譚金鋒%盧誌方%宋春雨%崔曉光
주립걸%왕현뢰%담금봉%로지방%송춘우%최효광
大鼠%肺移植%再灌注损伤%硫酸葡聚糖%补体
大鼠%肺移植%再灌註損傷%硫痠葡聚糖%補體
대서%폐이식%재관주손상%류산포취당%보체
Rats%Lung transplantation%Reperfusion injury%Dextran sulfate%Lcomplement
目的 研究硫酸葡聚糖减轻大鼠移植肺缺血再灌注损伤的作用及其机理.方法 雄性Wistar大鼠32只,建立大鼠原位左肺移植模型.采用随机数字表法将受鼠分为两组(n=8),硫酸葡聚糖组(实验组):肺移植时再灌注前给予硫酸葡聚糖10mg/kg,对照组给予等体积的生理盐水.再灌注后2h收集两组受鼠的移植肺和血液样本,检测动脉血氧合指数,移植肺湿干质量比(W/D),肺组织中髓过氧化物酶(MPO)活性,丙二醛(MDA)及内皮素-1(ET-1)含量,以及血清中肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)含量.并观察移植肺组织病理学改变和移植肺组织中补体C3c和C6的沉积情况.结果 再灌注后2h,实验组氧合指数较对照组明显升高(P＜0.05);两组间W/D无显著差异;实验组移植肺组织中MPO活性、MDA含量和ET-1含量均较对照组显著降低,血清中TNF-α、IL-8含量与对照组相比也明显降低,差异均有统计学意义(P＜0.05).实验组移植肺损伤评分为(4.53±0.46),对照组为(5.28±0.49),两组比较,差异无统计学意义(P＞0.05).实验组和对照组移植肺组织中补体C3c沉积评分分别为1.5±0.3和0.8±0.2,补体C6沉积评分分别为2.4±0.5和1.2±0.4,实验组均明显低于对照组(P＜0.05).结论 硫酸葡聚糖可以抑制移植肺组织中补体的沉积,减轻肺移植后的缺血再灌注损伤,改善移植肺的氧合功能,可能与减轻炎症和氧化损伤及保护内皮细胞有关.
目的 研究硫痠葡聚糖減輕大鼠移植肺缺血再灌註損傷的作用及其機理.方法 雄性Wistar大鼠32隻,建立大鼠原位左肺移植模型.採用隨機數字錶法將受鼠分為兩組(n=8),硫痠葡聚糖組(實驗組):肺移植時再灌註前給予硫痠葡聚糖10mg/kg,對照組給予等體積的生理鹽水.再灌註後2h收集兩組受鼠的移植肺和血液樣本,檢測動脈血氧閤指數,移植肺濕榦質量比(W/D),肺組織中髓過氧化物酶(MPO)活性,丙二醛(MDA)及內皮素-1(ET-1)含量,以及血清中腫瘤壞死因子-α(TNF-α)和白細胞介素-8(IL-8)含量.併觀察移植肺組織病理學改變和移植肺組織中補體C3c和C6的沉積情況.結果 再灌註後2h,實驗組氧閤指數較對照組明顯升高(P＜0.05);兩組間W/D無顯著差異;實驗組移植肺組織中MPO活性、MDA含量和ET-1含量均較對照組顯著降低,血清中TNF-α、IL-8含量與對照組相比也明顯降低,差異均有統計學意義(P＜0.05).實驗組移植肺損傷評分為(4.53±0.46),對照組為(5.28±0.49),兩組比較,差異無統計學意義(P＞0.05).實驗組和對照組移植肺組織中補體C3c沉積評分分彆為1.5±0.3和0.8±0.2,補體C6沉積評分分彆為2.4±0.5和1.2±0.4,實驗組均明顯低于對照組(P＜0.05).結論 硫痠葡聚糖可以抑製移植肺組織中補體的沉積,減輕肺移植後的缺血再灌註損傷,改善移植肺的氧閤功能,可能與減輕炎癥和氧化損傷及保護內皮細胞有關.
목적 연구류산포취당감경대서이식폐결혈재관주손상적작용급기궤리.방법 웅성Wistar대서32지,건립대서원위좌폐이식모형.채용수궤수자표법장수서분위량조(n=8),류산포취당조(실험조):폐이식시재관주전급여류산포취당10mg/kg,대조조급여등체적적생리염수.재관주후2h수집량조수서적이식폐화혈액양본,검측동맥혈양합지수,이식폐습간질량비(W/D),폐조직중수과양화물매(MPO)활성,병이철(MDA)급내피소-1(ET-1)함량,이급혈청중종류배사인자-α(TNF-α)화백세포개소-8(IL-8)함량.병관찰이식폐조직병이학개변화이식폐조직중보체C3c화C6적침적정황.결과 재관주후2h,실험조양합지수교대조조명현승고(P＜0.05);량조간W/D무현저차이;실험조이식폐조직중MPO활성、MDA함량화ET-1함량균교대조조현저강저,혈청중TNF-α、IL-8함량여대조조상비야명현강저,차이균유통계학의의(P＜0.05).실험조이식폐손상평분위(4.53±0.46),대조조위(5.28±0.49),량조비교,차이무통계학의의(P＞0.05).실험조화대조조이식폐조직중보체C3c침적평분분별위1.5±0.3화0.8±0.2,보체C6침적평분분별위2.4±0.5화1.2±0.4,실험조균명현저우대조조(P＜0.05).결론 류산포취당가이억제이식폐조직중보체적침적,감경폐이식후적결혈재관주손상,개선이식폐적양합공능,가능여감경염증화양화손상급보호내피세포유관.
Objective To investigate the effects of dextran sulfate on lung ischemia-reperfusion injury after lung transplantation in rats.Method A total of 32 male Wistar rats were subjected to unilateral left lung orthotopic transplantation.They were randomly divided two groups (n =16 each):DXS group [DXS (10 mg/kg) was given prior to the reperfusion],and the control group (the same volume of normal saline was given).After animals were sacrificed,the lung graft was harvested 2 h after reperfusion.Oxygenation indexes,wet/dry ratio (W/D),myeloperoxidase (MPO) activity,malondialdehyde (MDA) and endothelin 1 (ET-1) in the transplanted lung,and tumor necrosis factor a (TNF-α) and interleukin 8 (IL-8) in serum were measured.The lung injury scores were evaluated and complement deposition was observed.Result After 2-h reperfusion,compared to the control group,oxygenation indexes were improved significantly in DXS group (P＜0.05),but there were no significant differences in W/D between two groups.In DXS group,the activity of MPO was significantly reduced,and the contents of MDA and ET-1 in the lung tissue were significantly reduced as compared with the control group.DXS reduced the level of TNF-α and IL-8 markedly in serum (P ＜0.05).There was no significant difference in lung injury score between two groups (4.53 ± 0.46 vs.5.28 ±0.49,P＞0.05).Compared to the control group,DXS reduced the deposition of C3c (0.8 ±0.2vs1.5±0.3) andC6 (1.2±0.4vs.2.4±0.5) (P＜0.05).Conclusion Administration of DXS attenuated ischemia-reperfusion injury after lung transplantation by inhibiting complement deposition,and improved the oxygenation of the transplanted lung.This protection was associated with inhibition of inflammation and oxidation and endothelial cytoprotection.
