食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
22期
35-36,37
,共3页
金线莲%正交实验%总生物碱%微波提取
金線蓮%正交實驗%總生物堿%微波提取
금선련%정교실험%총생물감%미파제취
Anoectochilus%orthogonal experimental%total alkaloids%microwave extraction
采用微波提取的方法，从中药材金线莲中提取总生物碱，以盐酸麻黄碱为对照品，分光光度法测定了总生物碱含量。以总生物碱含量为考察指标，讨论提取料液比、提取功率、提取时间等因素对提取结果的影响。通过正交试验L9（33），对提取条件进行优化，得到微波提取的最佳工艺条件，即料液比为1∶15 g/mL，提取功率为616 W，提取时间为6 min。与传统的加热回流法对照，经F-检验法及t-检验法检验，测定结果没有显著性差异。实验表明，该法操作简便，大大缩短了提取时间，节约了人力、物力，提取效果令人满意，对金线莲生物碱的开发利用提供了借鉴。
採用微波提取的方法，從中藥材金線蓮中提取總生物堿，以鹽痠痳黃堿為對照品，分光光度法測定瞭總生物堿含量。以總生物堿含量為攷察指標，討論提取料液比、提取功率、提取時間等因素對提取結果的影響。通過正交試驗L9（33），對提取條件進行優化，得到微波提取的最佳工藝條件，即料液比為1∶15 g/mL，提取功率為616 W，提取時間為6 min。與傳統的加熱迴流法對照，經F-檢驗法及t-檢驗法檢驗，測定結果沒有顯著性差異。實驗錶明，該法操作簡便，大大縮短瞭提取時間，節約瞭人力、物力，提取效果令人滿意，對金線蓮生物堿的開髮利用提供瞭藉鑒。
채용미파제취적방법，종중약재금선련중제취총생물감，이염산마황감위대조품，분광광도법측정료총생물감함량。이총생물감함량위고찰지표，토론제취료액비、제취공솔、제취시간등인소대제취결과적영향。통과정교시험L9（33），대제취조건진행우화，득도미파제취적최가공예조건，즉료액비위1∶15 g/mL，제취공솔위616 W，제취시간위6 min。여전통적가열회류법대조，경F-검험법급t-검험법검험，측정결과몰유현저성차이。실험표명，해법조작간편，대대축단료제취시간，절약료인력、물력，제취효과령인만의，대금선련생물감적개발이용제공료차감。
Microwave extraction method, distilled water for the second solvent extraction from Chinese herbal medicines Anoectochilus from the total alkaloids, ephedrine hydrochloride as a reference substance, used by spectrophotometry to extract the alkaloid content was determined. To Anoectochilus in total alkaloid content of the indicators for the inspection to discuss the extraction of material than, from power, extraction time on the effects of alkaloids extracted. By orthogonal experimental L9(33) of the Anoectochilus total alkaloids in the extraction conditions to optimize the design, by microwave extraction Anoectochilus total alkaloids in the optimum conditions for A1B3C2, that was to feed than 1∶15 g/mL, To extract power 616 W, from time to 6 min. Contrasting with the traditional water bath extraction,and using F-test and t-test to check up the result,no significant difference was observed. The experiments showed that the method was simple, greatly reducing the extraction time, saving a human, material and satisfactory results in the future Anoectochilus the development and utilization of alkaloids for reference.
