CAJ | 학술논문

从脱脂米糠中提取的粗多糖SPK-1以及纯化精制多糖SPK-2、SPK-3、SPK-4具有显著生物活性，具有增强免疫调节的功能。从细胞因子的层次考察了米糠多糖SPK-1，2，3，4对小鼠脾脏淋巴细胞活性、ConA和LPS丝裂原诱导的脾脏淋巴细胞活化增殖以及LPS、ConA诱导脾脏淋巴细胞分泌IFN-γ，IL-2，IL-10，IL-12，IL-17等细胞因子的影响。研究发现，SPK-1，2，3，4在体外都有显著增强小鼠脾脏淋巴细胞活性，而且均有促进小鼠脾脏淋巴细胞分泌IFN-γ， IL-2以及抑制IL-10，IL-12，IL-17因子分泌的作用。
종탈지미강중제취적조다당SPK-1이급순화정제다당SPK-2、SPK-3、SPK-4구유현저생물활성，구유증강면역조절적공능。종세포인자적층차고찰료미강다당SPK-1，2，3，4대소서비장림파세포활성、ConA화LPS사렬원유도적비장림파세포활화증식이급LPS、ConA유도비장림파세포분비IFN-γ，IL-2，IL-10，IL-12，IL-17등세포인자적영향。연구발현，SPK-1，2，3，4재체외도유현저증강소서비장림파세포활성，이차균유촉진소서비장림파세포분비IFN-γ， IL-2이급억제IL-10，IL-12，IL-17인자분비적작용。
Crude polysaccharide SPK-1 and purification of polysaccharide SPK-2, SPK-3, SPK-4 were extracted from defatted rice bran. These polysaccharides have significant biological activity of enhancing immune regulation. The experiments on a cellular level investigated SPK-1,2,3,4 the influence on the mouse spleen lymphocyte activity, the mitogen and proliferation of spleen lymphocyte induced by ConA and LPS, and the levels of spleen lymphocyte secreting IFN-γ, IL-2, IL-10, IL-12, IL-17 cell factor induced by ConA and LPS. The study found that SPK-1, 2, 3, 4 have significantly effects of enhancing the activity of mouse spleen lymphocytes in vitro. They also can promote the secretion level of IFN-γ, IL-2 and inhibition of IL-10, IL-12, IL-17 in murine spleen lymphocytes.