CAJ | 학술논문

目的：采用高效液相色谱（HPLC）法测定不同产地芫花根皮中西瑞香素含量，确定何种产地芫花根皮中西瑞香素含量最高。方法采用 Prodigy 5u 100A C18柱（250 mm ×4.60 mm，5μm），流动相为甲醇－0.2%磷酸水溶液（55：45），流速为1.0 mL / min，柱温30℃，紫外检测波长346 nm，进样量20μL。结果西瑞香素质量浓度在0.5~20μg / mL 范围内与峰面积具有良好的线性关系（ r =0.9984），平均回收率为98.62%，RSD =4.08%( n =6)。不同产地芫花根皮药材中西瑞香素含量差异较大。结论该法可准确地对芫花根皮药材中有效成分西瑞香素进行定量定性测定，对于保证其内在质量和临床疗效具有重要的意义。
목적：채용고효액상색보（HPLC）법측정불동산지원화근피중서서향소함량，학정하충산지원화근피중서서향소함량최고。방법채용 Prodigy 5u 100A C18주（250 mm ×4.60 mm，5μm），류동상위갑순－0.2%린산수용액（55：45），류속위1.0 mL / min，주온30℃，자외검측파장346 nm，진양량20μL。결과서서향소질량농도재0.5~20μg / mL 범위내여봉면적구유량호적선성관계（ r =0.9984），평균회수솔위98.62%，RSD =4.08%( n =6)。불동산지원화근피약재중서서향소함량차이교대。결론해법가준학지대원화근피약재중유효성분서서향소진행정량정성측정，대우보증기내재질량화림상료효구유중요적의의。
Objective To establish a HPLC determination method for the daphnoretin content in the root bark from different habitats of Daphne genkwa in order to determine what habitat of the root bark of Daphne genkwa has the highest daphnoretin content. Methods The OSD column (250 mm × 4. 60 mm,5 μm) was adopted with the mobile phase of methanol - 0. 2% phosphoric acid aqueous solution (55 : 45) at a flow rate of 1. 0 mL / min, the column temperature was 30 ℃ and the UV detection wavelength was 346 nm. The sample size was 20 μL. Results Daphnoretin in the range of 0. 5 - 20 μg / mL had good linear relation( r = 0. 998 4), and the average recovery rate was 98. 62% ,RSD = 4. 08% ( n = 6). The daphnoretin content in the root bark of Daphne genkwa had large difference among the dif-ferent habitats. Conclusion This method can accurately perform the quantitative and qualitative determination of the daphnoretin content in the root bark of Daphne genkwa and has a great significance for ensuring its internal quality and clinical curative effect.