中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
23期
6-7,8
,共3页
张燕青%林雪玉%朱金平%费燕
張燕青%林雪玉%硃金平%費燕
장연청%림설옥%주금평%비연
伏立康唑%高效液相色谱法%血药浓度
伏立康唑%高效液相色譜法%血藥濃度
복립강서%고효액상색보법%혈약농도
voriconazole%HPLC%blood drug concentration
目的:建立测定人血清中伏立康唑浓度的高效液相色谱法,用于临床该药的治疗药物浓度监测。方法采用乙腈沉淀处理血清样品,采用高效液相色谱紫外检测法测定。以硝西泮为内标,色谱柱为 Hypersil ODS 柱(200 mm ×4.6 mm,5μm),流动相为25 mmol / L 磷酸二氢钾缓冲液-乙腈(32:68),流速为1.0 mL / min,柱温为35℃,波长为256 nm。结果伏立康唑质量浓度在0.3~12μg / mL 范围内与峰面积比线性关系良好( r =0.9993),低、中、高质量浓度回收率分别为(105.0±3.6)%,(101.9±3.3)%,(101.1±3.4)%;日内、日间精密度的 RSD 均小于5%,定量限为0.3μg / mL。结论该方法简便快速、准确,适用于伏立康唑血药浓度的临床监测。
目的:建立測定人血清中伏立康唑濃度的高效液相色譜法,用于臨床該藥的治療藥物濃度鑑測。方法採用乙腈沉澱處理血清樣品,採用高效液相色譜紫外檢測法測定。以硝西泮為內標,色譜柱為 Hypersil ODS 柱(200 mm ×4.6 mm,5μm),流動相為25 mmol / L 燐痠二氫鉀緩遲液-乙腈(32:68),流速為1.0 mL / min,柱溫為35℃,波長為256 nm。結果伏立康唑質量濃度在0.3~12μg / mL 範圍內與峰麵積比線性關繫良好( r =0.9993),低、中、高質量濃度迴收率分彆為(105.0±3.6)%,(101.9±3.3)%,(101.1±3.4)%;日內、日間精密度的 RSD 均小于5%,定量限為0.3μg / mL。結論該方法簡便快速、準確,適用于伏立康唑血藥濃度的臨床鑑測。
목적:건립측정인혈청중복립강서농도적고효액상색보법,용우림상해약적치료약물농도감측。방법채용을정침정처리혈청양품,채용고효액상색보자외검측법측정。이초서반위내표,색보주위 Hypersil ODS 주(200 mm ×4.6 mm,5μm),류동상위25 mmol / L 린산이경갑완충액-을정(32:68),류속위1.0 mL / min,주온위35℃,파장위256 nm。결과복립강서질량농도재0.3~12μg / mL 범위내여봉면적비선성관계량호( r =0.9993),저、중、고질량농도회수솔분별위(105.0±3.6)%,(101.9±3.3)%,(101.1±3.4)%;일내、일간정밀도적 RSD 균소우5%,정량한위0.3μg / mL。결론해방법간편쾌속、준학,괄용우복립강서혈약농도적림상감측。
Objective To establish a HPLC method to determine the concentration of voriconazole in human plasma for its clinical ap-plication in the curative drug concentration monitoring. Methods The serum sample was performed the precipitation treatment by acte-tonitrile, then HPLC - UV was adopted for detection. With nitrazepan as the internal standard, the chromatographic column was the Hy-persil ODS columm (200. 0 mm × 4. 6 mm, 5 μm), the mobile phase was 25 mmol / L potassium dihydrogen phosphate buffer solution -acetonitrile ( 32 : 68 ) . The detection wavelength was at 256 nm with a flow rate of 1 mL / min and the column temperature of 35 ℃ . Results The concentration of voriconazole in the range of 0. 3 - 12 μg / mL( r = 0. 999 3) showed the good linearity. The recovery rates of low, middle and high concentrations were (105. 0 ± 3. 6)% , (101. 9 ± 3. 3)% and (101. 1 ± 3. 4)% , respectively. The intra - day and inter - day RSD were both less than 5% . The limit of quantitation was 0. 3 μg / mL. Conclusion The established method is fast, accu-rate and suitable for the determination of blood voriconazole concentration.