CAJ | 학술논문

采用饥饿法聚合合成了3种不同配比的甲基丙烯酰氧乙基磷酰胆碱-甲基丙烯酸二元共聚物(poly(MPC-co-MA),PMA),并将其静电吸附在壳聚糖表面(CS-PMA)。通过动态接触角(DCA)对影响静电吸附改性的盐及聚合物组成因素进行研究。用 X-射线光电子能谱(XPS)、原子力显微镜(AFM)对改性壳聚糖膜的表面元素组成、形貌进行表征,并通过血小板黏附实验对其抗凝血性能进行评价。结果表明,这种利用静电吸附的方式将磷酰胆碱聚合物吸附在壳聚糖表面,可获得具有仿细胞外层膜结构的涂层表面。与壳聚糖相比,改性后壳聚糖可以显著降低对血小板的黏附,抗凝血性能显著提高。该研究为构建仿细胞外层膜结构改善材料血液相容性提供了一种简单、便捷的方法。
채용기아법취합합성료3충불동배비적갑기병희선양을기린선담감-갑기병희산이원공취물(poly(MPC-co-MA),PMA),병장기정전흡부재각취당표면(CS-PMA)。통과동태접촉각(DCA)대영향정전흡부개성적염급취합물조성인소진행연구。용 X-사선광전자능보(XPS)、원자력현미경(AFM)대개성각취당막적표면원소조성、형모진행표정,병통과혈소판점부실험대기항응혈성능진행평개。결과표명,저충이용정전흡부적방식장린선담감취합물흡부재각취당표면,가획득구유방세포외층막결구적도층표면。여각취당상비,개성후각취당가이현저강저대혈소판적점부,항응혈성능현저제고。해연구위구건방세포외층막결구개선재료혈액상용성제공료일충간단、편첩적방법。
Three random copolymers poly (2-methacryloyloxyethyl phosphorylcholine-co-methacrylic acid ) (PMAs)were synthesized by free radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and methacrylic acid (MA)with different monomer ratios under monomer-starved conditions.The synthesized PMA polyanions were assembled on chitosan (CS)film surfaces via electrostatic adsorption (CS-PMA).The modified surfaces were characterized by dynamic contact angle (DCA),X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM).The anticoagulation property of the surfaces was estimated by platelet ad-hesion measurements.The results indicated that cell outer membrane mimetic structures were formed on the modified surfaces with PMA and the anticoagulation property of the modified surfaces was significantly im-proved.This facile method of fabricating cell outer membrane mimetic surfaces may have potential applications in the fields of hemocompatible coatings,drug delivery,and tissue engineering.