中华乳腺病杂志（电子版）
中華乳腺病雜誌（電子版）
중화유선병잡지（전자판）
CHINESE JOURNAL OF BREAST DISEASE(ELECTRONIC VERSION)
2014年
5期
330-333
,共4页
姚春%邵力伟%郑刚%刘锦修%郜朝霞%任德发%伍龙
姚春%邵力偉%鄭剛%劉錦脩%郜朝霞%任德髮%伍龍
요춘%소력위%정강%류금수%고조하%임덕발%오룡
乳腺肿瘤%猬蛋白质类,人类%病理学,临床
乳腺腫瘤%猬蛋白質類,人類%病理學,臨床
유선종류%위단백질류,인류%병이학,림상
Breast neoplasms%Sonic hedgehog protein,human%Pathology,clinical
目的观察音猬因子( Shh)在乳腺癌组织中的表达及其临床意义。方法收集2008年1月至2012年12月在武汉市第五医院手术治疗的乳腺癌患者的乳腺癌组织和癌旁正常乳腺组织各46例。采用实时荧光定量 PCR法和免疫组织化学染色检测乳腺癌组织及癌旁正常乳腺组织中 Shh mRNA和Shh蛋白的表达,并探讨Shh蛋白与乳腺癌临床病理特征的关系。计数资料比较采用χ2检验,计量资料比较采用独立样本t检验或单因素方差分析。结果乳腺癌组织中Shh mRNA表达水平明显高于癌旁正常乳腺组织(1.54±0.47比0.60±0.38,t=-12.02,P=0.000),Shh蛋白阳性表达率也明显高于癌旁正常乳腺组织[69.6%(32/46)比17.4%(8/46),χ2=25.48,P=0.000],并且其表达水平与乳腺癌的临床分期密切相关,患者临床分期越晚,Shh蛋白表达水平越高[Ⅲ期+Ⅳ期：Ⅰ期+Ⅱ期：77.8%(28/36)比4/10,χ2=5.28,P=0.022]。结论 Shh信号在乳腺癌组织中被激活。 Shh异常表达可能与乳腺癌的发生、发展相关,其可以作为一个潜在的肿瘤标志物应用于临床。
目的觀察音猬因子( Shh)在乳腺癌組織中的錶達及其臨床意義。方法收集2008年1月至2012年12月在武漢市第五醫院手術治療的乳腺癌患者的乳腺癌組織和癌徬正常乳腺組織各46例。採用實時熒光定量 PCR法和免疫組織化學染色檢測乳腺癌組織及癌徬正常乳腺組織中 Shh mRNA和Shh蛋白的錶達,併探討Shh蛋白與乳腺癌臨床病理特徵的關繫。計數資料比較採用χ2檢驗,計量資料比較採用獨立樣本t檢驗或單因素方差分析。結果乳腺癌組織中Shh mRNA錶達水平明顯高于癌徬正常乳腺組織(1.54±0.47比0.60±0.38,t=-12.02,P=0.000),Shh蛋白暘性錶達率也明顯高于癌徬正常乳腺組織[69.6%(32/46)比17.4%(8/46),χ2=25.48,P=0.000],併且其錶達水平與乳腺癌的臨床分期密切相關,患者臨床分期越晚,Shh蛋白錶達水平越高[Ⅲ期+Ⅳ期：Ⅰ期+Ⅱ期：77.8%(28/36)比4/10,χ2=5.28,P=0.022]。結論 Shh信號在乳腺癌組織中被激活。 Shh異常錶達可能與乳腺癌的髮生、髮展相關,其可以作為一箇潛在的腫瘤標誌物應用于臨床。
목적관찰음위인자( Shh)재유선암조직중적표체급기림상의의。방법수집2008년1월지2012년12월재무한시제오의원수술치료적유선암환자적유선암조직화암방정상유선조직각46례。채용실시형광정량 PCR법화면역조직화학염색검측유선암조직급암방정상유선조직중 Shh mRNA화Shh단백적표체,병탐토Shh단백여유선암림상병리특정적관계。계수자료비교채용χ2검험,계량자료비교채용독립양본t검험혹단인소방차분석。결과유선암조직중Shh mRNA표체수평명현고우암방정상유선조직(1.54±0.47비0.60±0.38,t=-12.02,P=0.000),Shh단백양성표체솔야명현고우암방정상유선조직[69.6%(32/46)비17.4%(8/46),χ2=25.48,P=0.000],병차기표체수평여유선암적림상분기밀절상관,환자림상분기월만,Shh단백표체수평월고[Ⅲ기+Ⅳ기：Ⅰ기+Ⅱ기：77.8%(28/36)비4/10,χ2=5.28,P=0.022]。결론 Shh신호재유선암조직중피격활。 Shh이상표체가능여유선암적발생、발전상관,기가이작위일개잠재적종류표지물응용우림상。
Objective To detect the expression of sonic hedgehog ( Shh) in breast cancer tissue and its clinical significance. Methods Totally 46 patients with breast cancer who were treated by operation in Fifth Hospital of Wuhan City from January 2008 to December 2012 were enrolled. Their breast cancer tissues and normal para-carcinoma tissues were analyzed. The mRNA and Protein expressions of Shh in breast cancer tissues and normal paracarcinoma tissues were detected by real-time fluorescent quantitation PCR assay and immunohistochemistry and its relationship with clinicopathologic characteristics were also analyzed. Count data were compared using Chi-square test, and measurement data were compared with independent sample t test or One-Way ANOVA. Results The Shh mRNA level in breast cancer tissues was significantly higher than that in normal paracarcinoma tissues (1. 54±0. 47 vs 0. 60±0. 38,t=-12. 02,P=0. 000), and the positive rate of Shh protein in breast cancer tissues was significantly higher than that in normal paracarcinoma tissues [69. 6% (32/46) vs 17. 4% (8/46),χ2=25. 48,P=0. 000]. Shh protein expression was closely related to clinicopathologic stage. The more advanced stage, the higher Shh protein expression rate [ stage Ⅲ+Ⅳ : stageⅠ+Ⅱ=77. 8%(28/36) vs 4/10,χ2=5. 28,P=0. 022]. Conclusions The Shh signal is activated in breast cancer tissues and its abnormal expression may play an important role in the occurrence and development of breast cancer. Shh may be used as a potential tumor marker in clinic.
