CAJ | 학술논문

目的：观察大鼠弥漫性轴索损伤（ DAI）后Homer1蛋白的表达规律。方法：将45只SD大鼠随机分为对照组及DAI组，后者按DAI后处死大鼠的时间不同分为30min、1h、3h、6h、12h、24h、72h、7d组。建立大鼠DAI动物模型，运用免疫组织化学技术及图像分析方法观察脑组织Homer1蛋白的表达。结果：损伤后30min，Homer1蛋白染色阳性细胞数增多，染色增强（P＜0．05），随着损伤时间的延长，阳性细胞数及阳性表达范围逐渐扩大，于伤后24h达到高峰（P＜0．01），随后逐渐下降。对照组可见少量Homer1蛋白表达。结论：Homer1蛋白是大鼠DAI后的灵敏指标，在不同时间段表达具有时序性变化规律，对于早期诊断DAI及损伤时间的推断具有一定的参考价值。
목적：관찰대서미만성축색손상（ DAI）후Homer1단백적표체규률。방법：장45지SD대서수궤분위대조조급DAI조，후자안DAI후처사대서적시간불동분위30min、1h、3h、6h、12h、24h、72h、7d조。건립대서DAI동물모형，운용면역조직화학기술급도상분석방법관찰뇌조직Homer1단백적표체。결과：손상후30min，Homer1단백염색양성세포수증다，염색증강（P＜0．05），수착손상시간적연장，양성세포수급양성표체범위축점확대，우상후24h체도고봉（P＜0．01），수후축점하강。대조조가견소량Homer1단백표체。결론：Homer1단백시대서DAI후적령민지표，재불동시간단표체구유시서성변화규률，대우조기진단DAI급손상시간적추단구유일정적삼고개치。
Objective:The test is to investigate the expression of Homer 1 protein after diffusing axonal injury (DAI) in rats.Method:45 SD rats are going to be randomly divided into 8 DAI groups at 30min, 1h, 3h, 6h, 12h, 24h, 72h, 7d post-injury respectively and 1 control group.Based on Marmarou's impact/compression trauma model, the rat model of DAI will be established , Immunohistochemical method , as well as image analysis system , can be applied to observe the expression of Homer 1 protein in brain tissue.Result:After 30min, the amount of Ho-mer1 positive expression neurons has increased and positive staining has strengthened (P<0.05), and with the ex-tension of post-traumatic intervals, the expression keeps on increasing gradually , peaking at 24h(P<0.01), and then it has decreased gradually .The control group shows small amount of Homer 1 protein expression .Conclusion:Homer1 protein is a sensitive index to DAI and is helpful to diagnose early DAI .The time-dependent pattern of Ho-mer1 protein changes may be helpful for dating DAI .