水产学杂志
水產學雜誌
수산학잡지
CHINESE JOURNAL OF FISHERIES
2014年
6期
13-16
,共4页
薛淑群%孙源%孙中武%卢建国
薛淑群%孫源%孫中武%盧建國
설숙군%손원%손중무%로건국
黄颡鱼%遗传分化%TRAP分析
黃顙魚%遺傳分化%TRAP分析
황상어%유전분화%TRAP분석
Pelteobagrus fulvidraco%genetic differentiation%target region amplified polymorphism(TRAP) analysis
本文采用40对靶位区域扩增多态性(TRAP)标记分析了野生和养殖黄颡鱼Pelteobagrus fulvidraco群体的遗传多样性。从40对引物中共扩增出512个位点,其中多态位点278个,多态位点比例为54.3%。筛选出的40对引物的扩增产物都具有多态性,带型稳定,重复性好,可用于统计分析。 PopGene分析表明:野生黄颡鱼群体的遗传多样性高于养殖群体。群体间的Nei遗传分化系数和基因流指数Nm分别为0.6832和0.2318,表明两个群体间发生了明显的分化。群体间Nm值小于1,说明两群体发生的基因交流较少。
本文採用40對靶位區域擴增多態性(TRAP)標記分析瞭野生和養殖黃顙魚Pelteobagrus fulvidraco群體的遺傳多樣性。從40對引物中共擴增齣512箇位點,其中多態位點278箇,多態位點比例為54.3%。篩選齣的40對引物的擴增產物都具有多態性,帶型穩定,重複性好,可用于統計分析。 PopGene分析錶明:野生黃顙魚群體的遺傳多樣性高于養殖群體。群體間的Nei遺傳分化繫數和基因流指數Nm分彆為0.6832和0.2318,錶明兩箇群體間髮生瞭明顯的分化。群體間Nm值小于1,說明兩群體髮生的基因交流較少。
본문채용40대파위구역확증다태성(TRAP)표기분석료야생화양식황상어Pelteobagrus fulvidraco군체적유전다양성。종40대인물중공확증출512개위점,기중다태위점278개,다태위점비례위54.3%。사선출적40대인물적확증산물도구유다태성,대형은정,중복성호,가용우통계분석。 PopGene분석표명:야생황상어군체적유전다양성고우양식군체。군체간적Nei유전분화계수화기인류지수Nm분별위0.6832화0.2318,표명량개군체간발생료명현적분화。군체간Nm치소우1,설명량군체발생적기인교류교소。
Forty pairs of target region amplified polymorphism (TRAP) markers were applied to analyze genetic diversity between wild and cultured yellow catfish Pelteobagrus fulvidraco. There were 512 loci in 40 pairs of primers amplified, including 278 polymor-phic loci, with polymorphic loci rate of 54.3%. The amplified products of 40 pairs of primers amplified all showed polymorphism, sta-bility, and good reproducibility, and were used for statistical analysis. PopGene analysis revealed that the genetic diversity was higher in wild populations than that in the cultured population. Nei coefficient were 0.6832 in genetic differentiation and 0.2318 in gene flow, indicating that the two populations of yellow catfish have obvious differentiation, and less gene exchange in inter-population due to gene flow index Nm value of less than 1.