CAJ | 학술논문

目的：研究周围神经损伤后高迁移率族蛋白B1(high mobility group protein B1，HMGB1)在损伤神经远侧端的细胞定位及表达变化，探讨HMGB1对施万细胞(Schwann cells，SCs)迁移特性的影响，阐明HMGB1功能作用的细胞学基础。方法：建立Sprague-Dawley(SD)大鼠坐骨神经损伤模型，采用免疫荧光组织双标化学染色观察神经损伤后HMGB1的定位和表达情况，新生1～3 d SD大鼠取材体外培养SCs并进行纯化，利用Transwell小室检测HMGB1对SCs迁移的影响。结果：大鼠坐骨神经中HMGB1和S100共定位，主要定位于细胞核内，损伤后远侧端HMGB1表达明显升高；体外培养的SCs经过纯化后排列整齐、胞体透亮，纯度可达95%以上，可用于后续体外试验；HMGB1蛋白能够促进SCs迁移。结论：HMGB1参与损伤坐骨神经远侧端SCs的功能调节。
목적：연구주위신경손상후고천이솔족단백B1(high mobility group protein B1，HMGB1)재손상신경원측단적세포정위급표체변화，탐토HMGB1대시만세포(Schwann cells，SCs)천이특성적영향，천명HMGB1공능작용적세포학기출。방법：건립Sprague-Dawley(SD)대서좌골신경손상모형，채용면역형광조직쌍표화학염색관찰신경손상후HMGB1적정위화표체정황，신생1～3 d SD대서취재체외배양SCs병진행순화，이용Transwell소실검측HMGB1대SCs천이적영향。결과：대서좌골신경중HMGB1화S100공정위，주요정위우세포핵내，손상후원측단HMGB1표체명현승고；체외배양적SCs경과순화후배렬정제、포체투량，순도가체95%이상，가용우후속체외시험；HMGB1단백능구촉진SCs천이。결론：HMGB1삼여손상좌골신경원측단SCs적공능조절。
Objective:To investigate the localization and expression changes of high mobility group protein B1(HMGB1) fol-lowing sciatic nerve injury, and also to test the effect of HMGB1 on migration of Schwann cells(SCs) in attempt to clarify the functional basis of the cells. Methods:With the establishment of rat sciatic nerve injury model, immunohistochemistry method was used to study the localization and the expression changes of HMGB1. SCs were also isolated from newborn rat pups (with-in postnatal 1-3 d) and purified. Transwell was used to test the effect of HMGB1 on migration of SCs. Results: Immunohisto-chemistry demonstrated that HMGB1 colocalized with S100 positive cells in the distal segment with most of them colocalized in nucleus, the positive staining increased after sciatic nerve injury. The purified SCs were in regular morphology. Statistical data showed that purification of the cell reached up to 95%. Extracellular HMGB1 was able to promote the migration of SCs. Conclusion:HMGB1 is involved in the functional regulation of SCs after sciatic nerve injury.