北华大学学报（自然科学版）
北華大學學報（自然科學版）
북화대학학보（자연과학판）
JOURNAL OF BEIHUA UNIVERSITY(NATURAL SCIENCE)
2014年
6期
743-746
,共4页
黄连素%人脐静脉内皮细胞%内皮功能障碍%软脂酸%一氧化氮
黃連素%人臍靜脈內皮細胞%內皮功能障礙%軟脂痠%一氧化氮
황련소%인제정맥내피세포%내피공능장애%연지산%일양화담
berberine%HUVECs%endothelial dysfunciotn%palmitate%NO
目的：研究黄连素对软脂酸诱导人脐静脉内皮细胞( HUVECs)损伤的保护作用,并初步探讨其作用机制.方法采用500μmol/L软脂酸培养HUVECs 24 h,建立内皮细胞损伤模型,MTT法观察黄连素对细胞存活率的影响；检测细胞培养上清液中一氧化氮( NO)含量；RT-PCR法检测内皮型一氧化氮合酶( eNOS) mRNA水平；Western blot方法检测eNOS和磷酸化eNOS蛋白的表达.结果与对照组比较,软脂酸组细胞存活率降低( P<0.05),培养液中NO含量下降(P<0.05),细胞内eNOS mRNA水平、eNOS及磷酸化eNOS蛋白表达水平均显著下降(P<0.01,P<0.05).与软脂酸组比较,黄连素组细胞存活率增加,培养液中NO含量明显提高(P<0.05),细胞内eNOS mRNA水平和磷酸化蛋白表达水平均显著提高(P<0.01,P<0.05).结论黄连素对软脂酸引起的血管内皮细胞损伤有显著的保护作用,并可能与上调eNOS、促进NO生成有关.
目的：研究黃連素對軟脂痠誘導人臍靜脈內皮細胞( HUVECs)損傷的保護作用,併初步探討其作用機製.方法採用500μmol/L軟脂痠培養HUVECs 24 h,建立內皮細胞損傷模型,MTT法觀察黃連素對細胞存活率的影響；檢測細胞培養上清液中一氧化氮( NO)含量；RT-PCR法檢測內皮型一氧化氮閤酶( eNOS) mRNA水平；Western blot方法檢測eNOS和燐痠化eNOS蛋白的錶達.結果與對照組比較,軟脂痠組細胞存活率降低( P<0.05),培養液中NO含量下降(P<0.05),細胞內eNOS mRNA水平、eNOS及燐痠化eNOS蛋白錶達水平均顯著下降(P<0.01,P<0.05).與軟脂痠組比較,黃連素組細胞存活率增加,培養液中NO含量明顯提高(P<0.05),細胞內eNOS mRNA水平和燐痠化蛋白錶達水平均顯著提高(P<0.01,P<0.05).結論黃連素對軟脂痠引起的血管內皮細胞損傷有顯著的保護作用,併可能與上調eNOS、促進NO生成有關.
목적：연구황련소대연지산유도인제정맥내피세포( HUVECs)손상적보호작용,병초보탐토기작용궤제.방법채용500μmol/L연지산배양HUVECs 24 h,건립내피세포손상모형,MTT법관찰황련소대세포존활솔적영향；검측세포배양상청액중일양화담( NO)함량；RT-PCR법검측내피형일양화담합매( eNOS) mRNA수평；Western blot방법검측eNOS화린산화eNOS단백적표체.결과여대조조비교,연지산조세포존활솔강저( P<0.05),배양액중NO함량하강(P<0.05),세포내eNOS mRNA수평、eNOS급린산화eNOS단백표체수평균현저하강(P<0.01,P<0.05).여연지산조비교,황련소조세포존활솔증가,배양액중NO함량명현제고(P<0.05),세포내eNOS mRNA수평화린산화단백표체수평균현저제고(P<0.01,P<0.05).결론황련소대연지산인기적혈관내피세포손상유현저적보호작용,병가능여상조eNOS、촉진NO생성유관.
Objective To explore the protective effect of berberine on palmitate-induced injury in human umbilical vein endothelial cells ( HUVECs ) and reveal its mechanism. Method The endothelial cell injury model was established by culturing HUVECs with 500 μmol/L palmitate for 24 h. The cell viability of HUVECs was observed by MTT assays. The content of Nitric oxide ( NO ) in the supernatant was determined. The mRNA level of endothelial nitric oxide synthase(eNOS)was measured by RT-PCR,and the protein levels of eNOS and p-eNOS were analyzed by western blot. Results Compared with those in the control group,the cell viability of HUVECs, the content of NO in supernatant,mRNA level of eNOS,and the protein levels of eNOS and p-eNOS in palmitate group were significantly decreased(P<0. 01,P<0. 05). Compared with that in the palmitate group,the cell viability of HUVECs in the berberine group was increased. NO content,mRNA level of eNOS,and the protein levels of eNOS and p-eNOS in the berberine group were all significantly increased(P<0. 01 or P<0. 05). Conclusion Berberine has a significant protective effect on palmitate-induced endothelial cell injury,which might be related to the up-regulation of eNOS and the promotion of NO production.
