CAJ | 학술논문

目的：研究 C12联合检测多种肿瘤标志物对妇科恶性肿瘤诊断的意义。方法选取100例妇科良性病变、73例健康女性和病理确诊的81例妇科恶性肿瘤（卵巢癌、子宫颈癌、子宫内膜癌）病例的血清标本，利用 HD-2001A蛋白芯片仪进行12种肿瘤标志（包括 CA199，NSE，CEA，CA242，CA125，CA153，AFP，Ferritin，Free-PSA，PSA， HGH and β-HCG）的联合定量检测，对比 C12芯片检测系统检测妇科恶性肿瘤与良性病变及正常对照组的差异性。结果妇科恶性肿瘤组中 CA199、CEA 水平及阳性率均高于良性病变组和正常对照组，统计学差异显著（p<0.05）。卵巢癌中 CA199和 CA125、子宫颈癌和子宫内膜癌中 CEA 水平高于正常对照组，差异有显著性（p<0.05）。卵巢癌中 CA199和 CA125联合检测能提高阳性率。结论利用 C12联合检测多种肿瘤标志物可以提高恶性肿瘤诊断的准确性，其中卵巢癌中 CA199和 CA125、子宫颈癌和子宫内膜癌中 CEA 肿瘤标志物蛋白芯片检测对筛查妇科肿瘤高危人群具有重要意义。
목적：연구 C12연합검측다충종류표지물대부과악성종류진단적의의。방법선취100례부과량성병변、73례건강녀성화병리학진적81례부과악성종류（란소암、자궁경암、자궁내막암）병례적혈청표본，이용 HD-2001A단백심편의진행12충종류표지（포괄 CA199，NSE，CEA，CA242，CA125，CA153，AFP，Ferritin，Free-PSA，PSA， HGH and β-HCG）적연합정량검측，대비 C12심편검측계통검측부과악성종류여량성병변급정상대조조적차이성。결과부과악성종류조중 CA199、CEA 수평급양성솔균고우량성병변조화정상대조조，통계학차이현저（p<0.05）。란소암중 CA199화 CA125、자궁경암화자궁내막암중 CEA 수평고우정상대조조，차이유현저성（p<0.05）。란소암중 CA199화 CA125연합검측능제고양성솔。결론이용 C12연합검측다충종류표지물가이제고악성종류진단적준학성，기중란소암중 CA199화 CA125、자궁경암화자궁내막암중 CEA 종류표지물단백심편검측대사사부과종류고위인군구유중요의의。
The present study aims to explore the significance of the combined detection of multiple serum tumor-markers using protein biochip technique in patients with gynecological tumors. The serum levels of 12 tumor markers, including CA19-9, NSE ,CEA, CA242 ,CA125, CA153, AFP, ferritin, free-PSA, PSA, HGH and β-HCG were measured using the multiple-tumor marker protein chip system (HD-2001A) in 81 cases of gynecologic tumors(including ovarian cancer, cervical cancer, endometrial cancer), 100 cases of benign lesions and 73 cases of healthy adults. The differences among the gynecological malignancy group, benign lesions group and normal control group were analyzed. The serum levels and positive rates of CEA and CA199 in patients with gynecological malignancy were significantly higher than those in patients with benign lesions and healthy adults (p< 0.05). The serum levels of CA199 and CA125 in cases with ovarian cancer were significantly higher than those of the normal control group(p<0.05), and the serum levels of CEA in cases with uterine cervical cancer and endometrial carcinoma were also significantly higher than those of the normal control group(p<0.05). The combined detection of CA199 and CA125 can improve the positive rate of ovarian cancer. Combined detection of multiple serum tumor-markers using protein biochip technology can significantly increase the diagnostic sensitivity for gynecological malignancy. The combined detection of CA199 and CA125 play important roles in the positive detective rates in population with high risk of gynecological malignancy.