CAJ | 학술논문

目的：常年监测分析上海市南翔地区呼吸道感染儿童病毒病原学情况。方法采集2007年1月至2013年9月门诊急性呼吸道感染患儿4389例鼻咽分泌物，多重逆转录PCR法(Multiplex RT-PCR)检测9种呼吸道病毒，包括流感病毒(FLU)、副流感病毒(PIV)、呼吸道合胞病毒(RSV)、腺病毒(ADV)、人博卡病毒(HBOV)、人冠状病毒(Cov)、肠病毒(EV)、人偏肺炎病毒(HMPV)、鼻病毒(HRV)。同期采集无呼吸道感染症状儿童鼻咽分泌物标本123例，同时多重逆转录PCR法检测9种呼吸道病毒。结果4389例呼吸道感染患儿鼻咽分泌物标本中呼吸道病毒阳性检出率为34.8%(1526/4389)，前3位病毒依次为FLU 10.3%(453/4389)、RSV 7.3%(320/4389)和PIV 6.2%(274/4389)；2种及2种以上呼吸道病毒混合感染273例(6.2%)。不同年龄组病毒总检出率差异有统计学意义(χ2=41.91，P<0.001)，学龄组儿童检出率最低为23.4%，其余3组检出率均≥35.0%；RSV、HRV在婴儿组检出率均较高；FLU在学龄组儿童中检出率较高为13.6%。儿童喘息性疾病中存在较高的病毒检出率，其中RSV检出率14.8%(30/204)，其次为HBOV 13.8%(28/204)。同期采集无呼吸道感染症状儿童鼻咽分泌物标本123例，病毒检出率6.5%(8/123)，与呼吸道感染组病毒检出率比较，差异有统计学意义(χ2=42.60，P<0.001)。结论在连续7年的常年检测中，FLU、RSV在该地区儿童呼吸道感染性疾病中占重要地位。不同年龄组病毒检出率存在差异，婴幼儿呼吸道感染有较高的病毒检出率，RSV检出率较高；随年龄增长，总体病毒检出率下降，但流感病毒在大年龄组的检出率增高。
목적：상년감측분석상해시남상지구호흡도감염인동병독병원학정황。방법채집2007년1월지2013년9월문진급성호흡도감염환인4389례비인분비물，다중역전록PCR법(Multiplex RT-PCR)검측9충호흡도병독，포괄류감병독(FLU)、부류감병독(PIV)、호흡도합포병독(RSV)、선병독(ADV)、인박잡병독(HBOV)、인관상병독(Cov)、장병독(EV)、인편폐염병독(HMPV)、비병독(HRV)。동기채집무호흡도감염증상인동비인분비물표본123례，동시다중역전록PCR법검측9충호흡도병독。결과4389례호흡도감염환인비인분비물표본중호흡도병독양성검출솔위34.8%(1526/4389)，전3위병독의차위FLU 10.3%(453/4389)、RSV 7.3%(320/4389)화PIV 6.2%(274/4389)；2충급2충이상호흡도병독혼합감염273례(6.2%)。불동년령조병독총검출솔차이유통계학의의(χ2=41.91，P<0.001)，학령조인동검출솔최저위23.4%，기여3조검출솔균≥35.0%；RSV、HRV재영인조검출솔균교고；FLU재학령조인동중검출솔교고위13.6%。인동천식성질병중존재교고적병독검출솔，기중RSV검출솔14.8%(30/204)，기차위HBOV 13.8%(28/204)。동기채집무호흡도감염증상인동비인분비물표본123례，병독검출솔6.5%(8/123)，여호흡도감염조병독검출솔비교，차이유통계학의의(χ2=42.60，P<0.001)。결론재련속7년적상년검측중，FLU、RSV재해지구인동호흡도감염성질병중점중요지위。불동년령조병독검출솔존재차이，영유인호흡도감염유교고적병독검출솔，RSV검출솔교고；수년령증장，총체병독검출솔하강，단류감병독재대년령조적검출솔증고。
Absract: Objective To report the result of annual monitoring and analysis of nasopharyngeal virus in children with respiratory tract infections in Nanxiang, Shanghai District. Methods Nasopharyngeal secretions were collected from 4389 children with acute respiratory tract infection in outpatient department from January 2007 to September 2013, 9 common respiratory viruses were analyzed by Multiplex RT-PCR, including inlfuenza virus (FLU), parainlfuenza virus (PIV), respiratory syncytical virus (RSV) , adenovirus (ADV), human bocavirus(HBOV), human coronavirus(Cov), enterovirus(EV), human metapneumovirus(HMPV), and rhinovirus(HRV). The same analysis was done in 123 asymptomatic children during the same period. Results The positive rate of detected respiratory viruses in children with respiratory tract infections in nasopharyngeal secretions were 34.8% (1526/4389), including FLU 10.3% (453/4389), RSV 7.3% (320/4389), PIV 6.2%(274/4389), ADV 3.3%(146/4389), HBOV 2.7%(118/4389), EV 2.5%(110/4389), Cov 2.4%(105/4389), HRV 1.6%(72/4389), HMPV 1.5%(67/4389);two and more combined respiratory viral infection were found in 273 cases (6.2%). The virus detection <br> rate between age groups was signiifcantly different (χ2=41.91, P<0.001). The school-age group had the lowest positive rate of 23.4%and the positive rates in other three groups were all higher than 35.0%. The infant group had the higher positive rate of RSV and HRV. FLU detection rate in school-age group was 13.6%. Respiratory viruses in children with asthmatic disease has high detection rate. RSV infection rate was the highest 14.8%(30/204) in the asthmatic disease group, followed by HBOV 13.8% (28/204). In nasopharyngeal secretions of 123 asymptomatic children, virus-positive detection rate of 6.5% (8/123), which showed signiifcant difference from that in respiratory virus infection group (χ2=42.60, P<0.001). Conclusions In seven consecutive years of testing, the inlfuenza virus and respiratory syncytial virus play an important role in children with respiratory tract infections in this region. The detection rate of virus showed difference between different age groups and a higher detection rate of RSV in infants with respiratory tract infections was observed. The overall detection rate of virus was decreased with the increase of age excluding the inlfuenza virus.