CAJ | 학술논문

目的：观察DEX鞘内注射对剖宫产大鼠神经行为学及脾脏c-fos表达的影响。方法80只孕18d SD大鼠，随机分成5组，每组16只，CON组只进行麻醉，不进行剖宫产和鞘内注射；MOD组麻醉后进行剖宫产，进行鞘内注20μ生理盐水；DEX1，DEX2，DEX3组分别在麻醉后剖宫产大鼠，鞘内注射10μg/kg、30μg/kg、50μg/kg DEX，每组中的8只观察术前及术后12h、24h、48h右后肢PWT和PWL的变化。每组余下8只术后6h颈椎脱臼致死，取脾脏进行免疫组化检测c-fos表达。结果MOD组术后PWT、PWL较CON组明显降低(P<0.05)，逐渐升高(P<0.05)，DEX1、DEX2及DEX3组术后PWT、PWL较MOD组明显降低，和DEX注射剂量呈相关性，随着时间推移逐渐升高(P<0.05)。 DEX1、DEX2、DEX3之间也有明显差异(P<0.05)。MOD组脾脏c-fos表达较CON组明显升高(P<0.05)，鞘内注射DEX 后较MOD 组明显降低(P<0.05)，并呈剂量相关。结论DEX鞘内注射能明显改善剖宫产大鼠疼痛敏化，脾脏c-fos表达降低，呈剂量相关性。
목적：관찰DEX초내주사대부궁산대서신경행위학급비장c-fos표체적영향。방법80지잉18d SD대서，수궤분성5조，매조16지，CON조지진행마취，불진행부궁산화초내주사；MOD조마취후진행부궁산，진행초내주20μ생리염수；DEX1，DEX2，DEX3조분별재마취후부궁산대서，초내주사10μg/kg、30μg/kg、50μg/kg DEX，매조중적8지관찰술전급술후12h、24h、48h우후지PWT화PWL적변화。매조여하8지술후6h경추탈구치사，취비장진행면역조화검측c-fos표체。결과MOD조술후PWT、PWL교CON조명현강저(P<0.05)，축점승고(P<0.05)，DEX1、DEX2급DEX3조술후PWT、PWL교MOD조명현강저，화DEX주사제량정상관성，수착시간추이축점승고(P<0.05)。 DEX1、DEX2、DEX3지간야유명현차이(P<0.05)。MOD조비장c-fos표체교CON조명현승고(P<0.05)，초내주사DEX 후교MOD 조명현강저(P<0.05)，병정제량상관。결론DEX초내주사능명현개선부궁산대서동통민화，비장c-fos표체강저，정제량상관성。
Objective To observe the affects of dexmedetomidine intrathecally on neurobehavioral and expression of c-fos in spleen in cesarean section rats. Methods A total of 80 experimental rats pregnant 18 days were randomly divided into five groups with 16 rats in each group. No intervention except anesthesia in CON group,The CON plus cesarean section and 0.9% NS 20μl intrathecally in MOD group. Dexmedetomidine administered intrathecally before cesarean section to rats after anesthesia in DEX1 , DEX2,DEX3 groups,intrathecally administered DEX with 10μg/kg,30μg/kg,50μg/kg respectively. Observe the affects of DEX on PWT and PWL on right hind leg in eight rats of each group at preoperative and postoperative 12h,24h,48h. the remained eight rats each group were killed by cervical dislocation after 6h postoperative ,and take spleen for immunohistochemistry to detect c-fos expression. Results The PWT and PWL value were significantly reduced postoperative in MOD group (P<0.05),and increased gradually after cesarean section (P<0.05),controlled with MOD group,the PWT and PWL value in DEX1,DEX2 and DEX3 groups were decreased obviously,administration of DEX dose and PWT,PWL appeared related,increased gradually after cesarean section. The level of c-fos in spleen in MOD group was obviously higher than CON group,and the levels in DEX1,DEX2,DEX3 group were significantly lower than MOD group (P<0.05),and the level of c-fos in spleen was obviously different between DEX1 group and DEX2,DEX3 groups (P<0.05). and was related with dose of DEX. Conclusion Administered dexmedetomidine can obviously improve pain sensitization in the cesarean section rats,reduced c-fos expression in spleen,and is related with dose of DEX.