CAJ | 학술논문

目的：通过研究microRNA-155（miR-155）在类风湿关节炎（RA）患者外周血的表达水平，分析miR-155与疾病临床特征的相关性，探讨其在RA发病及疾病进展中的意义。方法抽取50例初发RA患者及36例健康对照组外周血分离PBMC（外周血单个核细胞），提取和纯化miRNA，实时定量PCR检测miR-155的表达，以小分子RNA U6作为内对照，对miR-155的表达量进行相对定量分析。同时收集RA患者有关的临床参数，统计分析miR-155的表达水平与RA合并临床参数的相关性。对RA活动组中，随机分为甲氨蝶呤（MTX）单药治疗组，肿瘤坏死因子（TNF）-α抑制剂单药治疗组，两组随访观察12周，收集患者治疗前后外周血。实时荧光定量PCR法检测RA患者治疗前后外周血miR-155的表达水平变化。结果 RA患者中PBMC中miR-155的表达水平较健康对照组明显升高，(P<0.01)；同时RA患者miR-155的表达与患者活动度有关，与肺间质病变等无明显相关，RA患者TNF-α抑制剂单药治疗组中，miR-155的表达较治疗前下降，而在甲氨蝶呤治疗组中无明显下降。结论 miR-155在RA的PBMC中异常高表达，且与RA的活动度相关，提示miR-155可能可作为RA诊断及RA活动的一个预测指标。 miR-155在RA的发病机制中可能参与了TNF-α的致炎过程。
목적：통과연구microRNA-155（miR-155）재류풍습관절염（RA）환자외주혈적표체수평，분석miR-155여질병림상특정적상관성，탐토기재RA발병급질병진전중적의의。방법추취50례초발RA환자급36례건강대조조외주혈분리PBMC（외주혈단개핵세포），제취화순화miRNA，실시정량PCR검측miR-155적표체，이소분자RNA U6작위내대조，대miR-155적표체량진행상대정량분석。동시수집RA환자유관적림상삼수，통계분석miR-155적표체수평여RA합병림상삼수적상관성。대RA활동조중，수궤분위갑안접령（MTX）단약치료조，종류배사인자（TNF）-α억제제단약치료조，량조수방관찰12주，수집환자치료전후외주혈。실시형광정량PCR법검측RA환자치료전후외주혈miR-155적표체수평변화。결과 RA환자중PBMC중miR-155적표체수평교건강대조조명현승고，(P<0.01)；동시RA환자miR-155적표체여환자활동도유관，여폐간질병변등무명현상관，RA환자TNF-α억제제단약치료조중，miR-155적표체교치료전하강，이재갑안접령치료조중무명현하강。결론 miR-155재RA적PBMC중이상고표체，차여RA적활동도상관，제시miR-155가능가작위RA진단급RA활동적일개예측지표。 miR-155재RA적발병궤제중가능삼여료TNF-α적치염과정。
Objective This study was to screen for the miRNA-155 expressed in peripheral blood mono nuclear cells (PBMC) of RA, investigate the correlation between the expression of miR-155 and the Clinical features of RA.To evaluate the function of miR-155 in RA, and explore the pathogenesis of RA. Methods PBMC was separated from the peripheral blood of 50 patients with RA and 36 healthy individuals. Total RNAs were isolated and miRNAs were purified. The levels of miR-155 was determined by quantitative reverse transcription PCR (qRT-PCR),using U6 as housekeeping control. The correlation between the expression level of miR-155 in PBMC and the of clinical features were analyzed. The group of RA activity were randomly divided into the methotrexate mono therapy group and TNF-αinhibitors mono therapy group,two groups were followed for 12 weeks,Real-time quantitative PCR used to detect the expression of miR-155 in PBMC of RA patients before and after the treatment. Results The expressions of PBMC miR-155 were higher in RA patients than those in the healthy control individuals (P<0.01);positive correla-tions were also observed between miR-155 expression and CRP,ESR,as well as DAS 28. No correlation was demonstrated be-tween miR-155 expression and the other clinical or laboratory parameters of the patients with RA ,including interstitial lung dis-ease. The expression of miR-155 was lower than before the treatment in the TNF-α inhibitors monotherapy group. Conclusion The expression of PBMC miR-155 was higher in RA patients and associated with RA patients ,activity. miR-155 may be potential non-invasive biomarkers for RA diagnosis and RA activity assessment.