CAJ | 학술논문

为了评价豫北地区泥鳅种质资源的多样性，以采自新乡地区泥鳅种群中的11个个体为材料，利用PCR技术扩增其线粒体16S rRNA和12S rRNA基因的部分序列，利用 Clustalw 2．0和DNAsp 4．10软件分析了不同个体间的差异和遗传多样性。结果表明：16S rRNA和12S rRNA基因种内个体间的差异分别为0．25％和0．97％，二者的分化程度较低；16S rRNA的进化速度约为12S rRNA基因的4倍。16S rRNA和12S rRNA种群群体的单倍型间平均遗传距离分别为0.0027、0.0016，单倍型多样度指数分别为0.873、0.327，核苷酸多样性指数分别为0.00265和0.00081，平均核苷酸差异数分别为1.636和0.327。可见，豫北泥鳅种群具有较低的遗传多样性。
위료평개예북지구니추충질자원적다양성，이채자신향지구니추충군중적11개개체위재료，이용PCR기술확증기선립체16S rRNA화12S rRNA기인적부분서렬，이용 Clustalw 2．0화DNAsp 4．10연건분석료불동개체간적차이화유전다양성。결과표명：16S rRNA화12S rRNA기인충내개체간적차이분별위0．25％화0．97％，이자적분화정도교저；16S rRNA적진화속도약위12S rRNA기인적4배。16S rRNA화12S rRNA충군군체적단배형간평균유전거리분별위0.0027、0.0016，단배형다양도지수분별위0.873、0.327，핵감산다양성지수분별위0.00265화0.00081，평균핵감산차이수분별위1.636화0.327。가견，예북니추충군구유교저적유전다양성。
In order to accurately evaluate the diversity of germplasm resources,to provide basic data and theoretical guidance for the genetic breeding of loach,the mtDNA 16S rRNA and 12S rRNA genes fragment of 1 1 individuals from the population of M.anguillicaudatus from the Xinxiang of Henan were amplified by PCR.The genetic diversity and sequence variation were studied via Using Clustalw 2.0 and DNAsp 4.10 software.These results indicated that the sequence variation among individuals were 0.9 7% and 0.2 5%.The genetic divergence was exceptionally low and the evolution rate of 16S rRNA was four times that of 12S rRNA.For the mtDNA 16S rRNA and 12S rRNA genes,the average genetic distance among the haplotypes in the population was 0.002 7 and 0.001 6,and the haplotype diversity was 0.873 and 0.327.The nucleotide diversity of the 11 individuals was 0.002 65 and 0.000 81,and the average number of nucleotide differences was 1.636 and 0.327.The results demonstrated that the genetic diversity of the population of M.anguillicaudatus from North Henan was very low and the protection for the population is necessary.