CAJ | 학술논문

根据已经克隆得到的金花茶（Camellia nitidissima Chi．）查尔酮合成酶基因序列，设计引物对P1／P2扩增出该基因全长并将其构建到原核表达载体 pGEX 4T 1中，转入大肠杆菌BL21后，用浓度为0．4 mmol／L的IPTG诱导其表达。SDS PAGE电泳检测结果显示，被诱导的大肠杆菌菌体中出现了约68 ku的特异融合蛋白，表明该基因在大肠杆菌中成功表达。
근거이경극륭득도적금화다（Camellia nitidissima Chi．）사이동합성매기인서렬，설계인물대P1／P2확증출해기인전장병장기구건도원핵표체재체 pGEX 4T 1중，전입대장간균BL21후，용농도위0．4 mmol／L적IPTG유도기표체。SDS PAGE전영검측결과현시，피유도적대장간균균체중출현료약68 ku적특이융합단백，표명해기인재대장간균중성공표체。
Full length gene fragment of chalcone synthase from Camellia nitidissima was cloned by primers P1 and P2 which were designed according to the sequence of CnCHS.The CnCHS gene was connected to prokaryotic expression vector pGEX 4T 1 and induced by 0.4 mmol/L IPTG after the recombinant plasmid was transferred to Escherichia coli BL2 1 .SDS PAGE results showed that a special band with molecular weight of 68 ku appeared,indicating that the CnCHS gene was expressed successfully in E.coli BL2 1 .