CAJ | 학술논문

为了解不同类型芥菜变种间的遗传多样性，利用66对 SSR引物对16份芥菜变种进行检测。结果表明，共扩出602个条带，多态性条带占92.5％，多态信息量（PIC）变幅为0.56～0.97，平均值为0.91。遗传相似系数为0.41～0.75，平均值为0.60，当相似系数为0.61时，可将供试材料分成4类。薹芥和抱子芥均单独为一类，第3类包括笋子芥、小叶芥和叶瘤芥，第4类包括白花芥、宽柄芥、长柄芥、大叶芥、凤尾芥、分蘖芥、卷心芥、茎瘤芥、花叶芥、结球芥和大头芥。表明16个芥菜变种间存在一定的遗传多样性。通过主成分分析也将供试材料分成4大类群，2种聚类分析结果基本一致，都说明来自相同省份的绝大部分材料聚在一起，呈现出一定的地域性分布规律，但聚类结果与芥菜形态没有必然联系。
위료해불동류형개채변충간적유전다양성，이용66대 SSR인물대16빈개채변충진행검측。결과표명，공확출602개조대，다태성조대점92.5％，다태신식량（PIC）변폭위0.56～0.97，평균치위0.91。유전상사계수위0.41～0.75，평균치위0.60，당상사계수위0.61시，가장공시재료분성4류。대개화포자개균단독위일류，제3류포괄순자개、소협개화협류개，제4류포괄백화개、관병개、장병개、대협개、봉미개、분얼개、권심개、경류개、화협개、결구개화대두개。표명16개개채변충간존재일정적유전다양성。통과주성분분석야장공시재료분성4대류군，2충취류분석결과기본일치，도설명래자상동성빈적절대부분재료취재일기，정현출일정적지역성분포규률，단취류결과여개채형태몰유필연련계。
In order to understand the genetic diversity of different mustard varieties,the denetic diversity of 1 6 mustard varieties was studied using 6 6 pairs of SSR markers in Brassica.The results showed that a total of 602 alleles were detected,with 92.5% polymorphism;the polymorphism information content (PIC)of SSR marker varied from 0.5 6 to 0.9 7 with an average of 0.9 1;the genetic similarity coefficient ranged from 0.41 to 0.75 with an average of 0.60,when the genetic similarity coefficient was 0.61,the 1 6 mustard varieties were divided into four clusters.B.juncea var.utilis Li and B.juncea var. gemmifera Lee et Li belonged to cluster 1 and cluster 2,respectively.B.juncea var.carassicaulis Chen et Yang,B.juncea var.foliosa Bailey,and B.juncea var.strumata Tsen et Lee belonged to cluster 3 . While B.juncea var.leucanthus Chen et Yang,B.juncea var.latipa Li,B.juncea var.longepetiolata Yang et Chen,B.juncea var.rugosa Bailey,B.juncea var.linearifolia Sun,B.juncea var.multiceps Tsen et Lee,B.juncea var.involuta Yang et Chen,B.juncea var.tumida Tsen et Lee,B.juncea var. multisecta Bailey,B.juncea var.capitata Hort,and B.juncea var.megarrhiza Tsen et Lee belonged to cluster 4.The results indicated that extensive genetic diversity existed among the 16 mustard varieties. Clustering analysis and principal component analysis both separated the 1 6 mustard varieties into four clusters,which was not fully in agreement with the taxonomic classification based on different edible organs of vegetable mustards,showing that the mustard varieties with the same source tended to be clustered into one subgroup,presenting a certain regional distribution rules,but there was no relationship between the clustering results and the mustard morphology.