CAJ | 학술논문

异羟肟酸及其衍生物是广泛存在于玉米等禾本科作物中的一种广谱抗性物质，丁布（DIM-BOA）是玉米植株中含量最大的异羟肟酸。为了明确不同丁布含量的玉米品种对亚洲玉米螟危害和生长发育的影响，采用高效液相色谱法测定了11个玉米品种五叶期的丁布含量；室内条件下在玉米叶片上人工接虫，调查亚洲玉米螟在11个玉米品种上的危害程度；用丁布含量依次降低的登海662、浚单20、隆玉602品种（P＜0.05）叶片饲喂亚洲玉米螟初孵幼虫，探讨不同含量丁布胁迫下亚洲玉米螟生长发育的变化。结果表明，不同玉米品种间丁布含量差异显著，登海662含量最高，德单5号含量最低。接种亚洲玉米螟后，丁布含量较低的德单5号、隆玉602、新单36、金骆驼335品种的花叶指数均大于40％，随着玉米品种丁布含量的增加，玉米螟的危害程度逐渐减轻。亚洲玉米螟幼虫发育历期和幼虫＋蛹总历期在取食3个玉米品种处理之间具有差异，其中取食隆玉602的幼虫发育历期和总发育历期最短，取食浚单20的处理最长。取食3个玉米品种的亚洲玉米螟均不能正常交配产卵和完成世代周期，其化蛹率、蛹质量和羽化率均随玉米品种丁布含量的降低而升高。
이간우산급기연생물시엄범존재우옥미등화본과작물중적일충엄보항성물질，정포（DIM-BOA）시옥미식주중함량최대적이간우산。위료명학불동정포함량적옥미품충대아주옥미명위해화생장발육적영향，채용고효액상색보법측정료11개옥미품충오협기적정포함량；실내조건하재옥미협편상인공접충，조사아주옥미명재11개옥미품충상적위해정도；용정포함량의차강저적등해662、준단20、륭옥602품충（P＜0.05）협편사위아주옥미명초부유충，탐토불동함량정포협박하아주옥미명생장발육적변화。결과표명，불동옥미품충간정포함량차이현저，등해662함량최고，덕단5호함량최저。접충아주옥미명후，정포함량교저적덕단5호、륭옥602、신단36、금락타335품충적화협지수균대우40％，수착옥미품충정포함량적증가，옥미명적위해정도축점감경。아주옥미명유충발육력기화유충＋용총력기재취식3개옥미품충처리지간구유차이，기중취식륭옥602적유충발육력기화총발육력기최단，취식준단20적처리최장。취식3개옥미품충적아주옥미명균불능정상교배산란화완성세대주기，기화용솔、용질량화우화솔균수옥미품충정포함량적강저이승고。
Hydroxamic acid and its derivatives,widely existing in gramineous crops such as maize,are a group of compounds with a broad spectrum of biological activities.DIMBOA(2 ,4-dihydroxy-7-methoxy-1 ,4-benzoxazin-3-one)is the dominant hydroxamic acid in maize.In the present study,high performance liquid chromatography(HPLC)was applied to qualitatively analyse the content of DIMBOA in 1 1 maize varieties;In the indoor test condition,newly hatched larvae of Ostrinia furnacalis were put on the maize leaves artificially,and the damaged degree of 1 1 maize varieties was investigated after several days;In order to investigate the changes in the growth of O.furnacalis under different degree of DIMBOA stress, Denghai 662,Xundan 20,Longyu 602 maize varieties with different levels of DIMBOA were applied to rear newly-hatched larvae.The results revealed that there was significant difference in DIMBOA content of different maize varieties,the DIMBOA content of Denghai 6 6 2 was the highest,and the lowest was Dedan No.5.The varieties with index of mosaic beyond 40% were Dedan No.5,Longyu 602,Xindan 36, Jinluotuo 335,which have lower DIMBOA content.With the increase of DIMBOA content in different maize varieties,the damaged degree of maize gradually decreased.The developmental duration of O.furnacalis larvae and total duration of larvae and pupae were significantly different among three maize varieties.The larvae duration and total duration on Longyu 602 were the shortest,and on Xundan 20 were the longest.O.furnacalis could not complete generation cycle on Denghai 662,Xundan 20,and Longyu 602,but the pupation rate,pupal weight and eclosion rate increased gradually with the decrease of DIMBOA content in the maize varieties.