CAJ | 학술논문

建立了高效阴离子交换色谱-脉冲安培电化学检测法同时测定生物转化样品中海藻糖、葡萄糖和麦芽糖的分析方法。选用 CarboPacTM10色谱柱（250 mm×2 mm）对分离条件进行优化，使用标准样品测定了线性范围和工作曲线，柱温为30℃，流速为0.30 mL／min，以氢氧化钠溶液和醋酸钠溶液为流动相进行梯度洗脱，脉冲安培法进行检测。研究结果表明，该方法可在15 min内实现海藻糖生物转化液中3种糖的快速定量分析。海藻糖、葡萄糖和麦芽糖峰面积与质量浓度的线性关系良好，检出限为0.010~0.100 mg／L。将此方法用于酶法制备海藻糖的检测，加标回收率为89.40％~103.2％。在生物转化样品中检测到海藻糖浓度为101.084 g／L，转化率达到了50.5％。该方法灵敏度高，简便快速，可应用于海藻糖制备样品中各种成分的分离和定量检测。
건립료고효음리자교환색보-맥충안배전화학검측법동시측정생물전화양품중해조당、포도당화맥아당적분석방법。선용 CarboPacTM10색보주（250 mm×2 mm）대분리조건진행우화，사용표준양품측정료선성범위화공작곡선，주온위30℃，류속위0.30 mL／min，이경양화납용액화작산납용액위류동상진행제도세탈，맥충안배법진행검측。연구결과표명，해방법가재15 min내실현해조당생물전화액중3충당적쾌속정량분석。해조당、포도당화맥아당봉면적여질량농도적선성관계량호，검출한위0.010~0.100 mg／L。장차방법용우매법제비해조당적검측，가표회수솔위89.40％~103.2％。재생물전화양품중검측도해조당농도위101.084 g／L，전화솔체도료50.5％。해방법령민도고，간편쾌속，가응용우해조당제비양품중각충성분적분리화정량검측。
An analytical method for the determination of trehalose,maltose,and glucose in biotransformation samples was developed by using high performance anion exchange chroma-tography coupled with pulsed ampere detection( HPAEC-PAD). The analysis was performed on a CarboPacTM 10 column( 250 mm × 2 mm ) with the gradient elution of NaOH-NaAc as the mobile phase. The column temperature was set at 30 ℃,the flow rate was 0. 30 mL/min. The results showed that trehalose,maltose,and glucose in biotransformation system were com-pletely separated and determined in 15 min. The linear ranges and the working curves were determined by using standard samples. The correlation coefficients of three kinds of carbohy-drates were over 0. 999 8 . The detection limits( LODs)were 0. 010-0. 100 mg/L. Under the optimized separation conditions,the recoveries of saccharides in the transformation system at three different spiked levels ranged from 89. 4% to 103. 2%. In biotransformation system,50 IU trehalose synthase were added into 200 g/L maltose for reaction of 8 h at 37 ℃,pH 8. 0. Under the above conditions,the concentration of trehalose in biotransformation sample was 101. 084 g/L,and the conversion rate of trehalose reached 50. 5%. The method can be applied to deter-mine the composition in the transformation system with the advantages of simplicity and con-venience.