CAJ | 학술논문

采用新型的介孔碳材料作为固载酪氨酸酶的检测平台构建生物传感器，应用于水体环境中苯酚污染物的检测，并通过高效液相色谱法对电化学酪氨酸酶生物传感器法的准确性进行了评价。研究表明，介孔碳的“空间限制效应”能够防止酪氨酸酶（三维尺寸为6.5 nm×9.8 nm×5.5 nm）体外去折叠失活。基于介孔碳材料构建的电化学酪氨酸酶生物传感器在苯酚污染物检测方面显示了优良的性能，其重现性、灵敏度、稳定性、选择性以及检出限均比较令人满意。基于介孔碳的电化学酪氨酸酶生物传感器对苯酚污染物的检出限达到20 nmol／L，线性范围为0.1~10μmol／L。采用基于介孔碳的电化学酪氨酸酶生物传感器和高效液相色谱法对实际水样品进行测定结果比对，结果表明该生物传感器方法检测结果准确、有效，适合于苯酚污染物突发污染事件的应急检测。
채용신형적개공탄재료작위고재락안산매적검측평태구건생물전감기，응용우수체배경중분분오염물적검측，병통과고효액상색보법대전화학락안산매생물전감기법적준학성진행료평개。연구표명，개공탄적“공간한제효응”능구방지락안산매（삼유척촌위6.5 nm×9.8 nm×5.5 nm）체외거절첩실활。기우개공탄재료구건적전화학락안산매생물전감기재분분오염물검측방면현시료우량적성능，기중현성、령민도、은정성、선택성이급검출한균비교령인만의。기우개공탄적전화학락안산매생물전감기대분분오염물적검출한체도20 nmol／L，선성범위위0.1~10μmol／L。채용기우개공탄적전화학락안산매생물전감기화고효액상색보법대실제수양품진행측정결과비대，결과표명해생물전감기방법검측결과준학、유효，괄합우분분오염물돌발오염사건적응급검측。
A novel electrochemical tyrosinase biosensor based on ordered graphitized meso-porous carbon( GMC)was obtained,which was used as a platform for phenol detection. The accuracy of tyrosinase biosensor method was comparatively evaluated by high performance liquid chromatography(HPLC). By entrapping tyrosinase molecules(6. 5 nm×9. 8 nm×5. 5 nm) into the mesopores of GMC( diameter 10 nm,GMC10),the“interspace confinement effect”of GMC10 may improve the stability of tyrosinase in vitro. After 21-day storage,the GMC10-based tyrosinase biosensor retained more than 85% of its initial response. It is indicated that GMC10 with“interspace confinement effect”can significantly prolong the life of tyrosinase molecules in vitro. Furthermore, the GMC-based tyrosinase biosensor displayed excellent analytical performances for phenol detection,such as stability,repeatability,selectivity,sensitivity and limit of detection. The GMC-based tyrosinase biosensor demonstrated a linear response for phenol from 0. 1 to 10 μmol/L with a low detection limit of 20 nmol/L. The comparative study between HPLC and GMC-based tyrosinase biosensor showed that the detection of phenol in water sample by the GMC-based tyrosinase biosensor method is reliable,accurate and effective. The proposed GMC-based tyrosinase biosensor proved to be a very promising“pre-alarm”tool for rapid detecting phenol pollution in emergency accidents.