中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
21期
35-36,37
,共3页
任卫琼%李珊%陈迎春%欧阳荣
任衛瓊%李珊%陳迎春%歐暘榮
임위경%리산%진영춘%구양영
复方天麻止痛颗粒%薄层色谱法%高效液相色谱法%质量标准
複方天痳止痛顆粒%薄層色譜法%高效液相色譜法%質量標準
복방천마지통과립%박층색보법%고효액상색보법%질량표준
Compound Gastrodia Elata Analgesic Granules%TLC%HPLC:quality standard
目的：制订复方天麻止痛颗粒的质量标准。方法采用薄层色谱（TLC）法鉴别方中川芎、桃仁、赤芍及红花；采用高效液相色谱法（HPLC）测定天麻素的含量，色谱条件为Hyperisil ODS C18柱（200 mm ×4.6 mm，5μm），流动相为甲醇－0.1%磷酸（11：89），流速1.0 mL/min，检测波长222 nm，柱温30℃。结果 TLC色谱中斑点清晰，易于识别。HPLC法精密度、重现性良好，天麻素进样量在0.08~0.44μg范围内与峰面积具有良好的线性关系，平均回收率为98.13%，RSD=0.77%( n=6)。结论该方法准确、可靠，可有效控制复方天麻止痛颗粒的质量。
目的：製訂複方天痳止痛顆粒的質量標準。方法採用薄層色譜（TLC）法鑒彆方中川芎、桃仁、赤芍及紅花；採用高效液相色譜法（HPLC）測定天痳素的含量，色譜條件為Hyperisil ODS C18柱（200 mm ×4.6 mm，5μm），流動相為甲醇－0.1%燐痠（11：89），流速1.0 mL/min，檢測波長222 nm，柱溫30℃。結果 TLC色譜中斑點清晰，易于識彆。HPLC法精密度、重現性良好，天痳素進樣量在0.08~0.44μg範圍內與峰麵積具有良好的線性關繫，平均迴收率為98.13%，RSD=0.77%( n=6)。結論該方法準確、可靠，可有效控製複方天痳止痛顆粒的質量。
목적：제정복방천마지통과립적질량표준。방법채용박층색보（TLC）법감별방중천궁、도인、적작급홍화；채용고효액상색보법（HPLC）측정천마소적함량，색보조건위Hyperisil ODS C18주（200 mm ×4.6 mm，5μm），류동상위갑순－0.1%린산（11：89），류속1.0 mL/min，검측파장222 nm，주온30℃。결과 TLC색보중반점청석，역우식별。HPLC법정밀도、중현성량호，천마소진양량재0.08~0.44μg범위내여봉면적구유량호적선성관계，평균회수솔위98.13%，RSD=0.77%( n=6)。결론해방법준학、가고，가유효공제복방천마지통과립적질량。
Objective To formulate the quality standard of Compound Gastrodia Elata Analgesic Granules. Methods The TLC method was adopted to identify the effective components in this preparation. The gastrodin content was detected by HPLC. The chromatographic conditions were the Hyperisil ODS column ( 200 mm × 4. 6 mm, 5μm ) , the mobile phase of methanol-0. 1% phosphoric acid ( 11:89 ) , a flow rate of 1. 0 mL/min, the detection wavelength of 222 nm and the column temperature of 30℃. Results The TLC spots were clear and easy to be recognized; the HPLC method had good precision and good reproducibility. The linear range of gastrodin was 0. 08-0. 44μg, the average recovery rate was 98. 13%, RSD=0. 77%( n=6 ) . Conclusion This method is accurate, reliable and can effectively control the quality of Compound Gastrodia Elata Analgesic Granules.
