CAJ | 학술논문

目的：建立测定暖宫孕子丸浸膏中黄芩苷含量的反相高效液相色谱法。方法以70%甲醇为溶剂，加热回流30 min提取。采用C18柱（150 mm ×4.6 mm，5μm )为分析柱，流动相为甲醇－0.4%磷酸(50：50)，检测波长为280 nm，柱温30℃。结果黄芩苷的进样量在0.057147~0.57147μg范围内与峰面积线性关系良好（ r=0.9999)；平均回收率为100.57%，RSD为1.21%( n=6）。结论该方法快速简便、准确可靠，重现性好，结果稳定，可用于暖宫孕子丸浸膏中黄芩药材的质量控制。
목적：건립측정난궁잉자환침고중황금감함량적반상고효액상색보법。방법이70%갑순위용제，가열회류30 min제취。채용C18주（150 mm ×4.6 mm，5μm )위분석주，류동상위갑순－0.4%린산(50：50)，검측파장위280 nm，주온30℃。결과황금감적진양량재0.057147~0.57147μg범위내여봉면적선성관계량호（ r=0.9999)；평균회수솔위100.57%，RSD위1.21%( n=6）。결론해방법쾌속간편、준학가고，중현성호，결과은정，가용우난궁잉자환침고중황금약재적질량공제。
Objective To establish the quantitative analysis method for determining the content of baicalin in Nuangong Yunzi Pill Ex-tract by RP-HPLC so as to provide the quality standard for the medicinal material in this preparation. Methods With 70% methanol as solvent, the extraction was conducted by heating reflux for 30 min. The C18 column ( 150 mm × 4. 6 mm, 5 μm ) was adopted with methanol-0. 4% phosphoric acid ( 50:50 ) as the mobile phase;the detection wavelength was 280 nm and the column temperature was 30℃. Results Under the above chromatographic conditions, the injection volume of baicalin in the range of 0. 057 147-0. 571 47 μg ( r=0. 999 9 ) showed a good linear relationship with the peak area;the average recovery rate of baicalin was 100. 57%, RSD=1. 21%( n=6). Conclusion This method is rapid, simple, accurate and reliable with good reproducibility and stable results and can be used for the quality control of Radix Scutellariae medical material in Nuangong Yunzi Pill Extract.