CAJ | 학술논문

以荷叶为原料，提取出荷叶多糖，经过脱蛋白、脱色初步纯化，进行抗氧化性及抑菌功能研究。采用羟基自由基和超氧阴离子自由基，对荷叶多糖的抗氧化性进行研究。实验结果表明，荷叶多糖对羟自由基、超氧自由基均有明显的清除作用，且最高清除率分别为95.8%、70.8%，同时其清除率随多糖浓度增加而增大。荷叶多糖对动植物油的抗氧化性有一定的效果，比酒石酸、柠檬酸稍强，但明显比VC弱。抑菌实验表明荷叶多糖对枯草芽孢杆菌、大肠杆菌均有一定的抑制作用，而对酵母、黑曲霉的抑制作用都不明显。最小抑菌质量浓度MIC在10 mg/mL～40 mg/mL之间。
이하협위원료，제취출하협다당，경과탈단백、탈색초보순화，진행항양화성급억균공능연구。채용간기자유기화초양음리자자유기，대하협다당적항양화성진행연구。실험결과표명，하협다당대간자유기、초양자유기균유명현적청제작용，차최고청제솔분별위95.8%、70.8%，동시기청제솔수다당농도증가이증대。하협다당대동식물유적항양화성유일정적효과，비주석산、저몽산초강，단명현비VC약。억균실험표명하협다당대고초아포간균、대장간균균유일정적억제작용，이대효모、흑곡매적억제작용도불명현。최소억균질량농도MIC재10 mg/mL～40 mg/mL지간。
In this paper, polysaccharide in lotus leaf were extracted. The polysaccharide was assessed for antioxidant and antimicrobial activities after preliminary purification with deproteinization and decoloration. Using the hydroxyl radical system and superoxide radical system, the antioxidant activities of lotus leaf polysaccharide is studied. The results showed that the polysaccharides in lotus left could significantly decrease the hydroxyl radicals and superoxide radical and the rate could arrived at 95.8 percent and 70.8 percent respectively, at the same time its clearance increases with the increase of polysaccharide concentrations. The polysaccharide from Lotus leaf on oxidation resistance has certain effect on animal and plant oil, a little stronger than tartaric acid, citric acid, but obviously weaker than VC. Lotus leaf polysaccharide was found that the polysaccharides could inhibit the growth of Escherichia coli and Bacillus subtilis. However, the Polysaccharides had little inhibitory effects on yeast and Aspergillus niger. The minimal inhibitory concentrations(MIC) varied from 10 mg/mL to 40 mg/mL.