CAJ | 학술논문

根据酿酒酵母2-10515摇瓶发酵生产谷胱甘肽发酵曲线特点，设计了一种低pH环境，即将种子液先在pH3.5条件下培养6 h，经接种后以适应发酵过程中长时间的低pH环境从而促进其合成谷胱甘肽的能力。低pH环境中的种子液接种发酵过程中，发酵液生物量、胞内谷胱甘肽总产量及胞内谷胱甘肽含量最大值分别为9.7 g/L、25.6 mg/L、4.1 mg/g，比未经处理条件下最大值分别提高了38.3%、39.5%、5.8%。此结果表明，利用低pH处理能够提高酿酒酵母2-10515生产谷胱甘肽能力。
근거양주효모2-10515요병발효생산곡광감태발효곡선특점，설계료일충저pH배경，즉장충자액선재pH3.5조건하배양6 h，경접충후이괄응발효과정중장시간적저pH배경종이촉진기합성곡광감태적능력。저pH배경중적충자액접충발효과정중，발효액생물량、포내곡광감태총산량급포내곡광감태함량최대치분별위9.7 g/L、25.6 mg/L、4.1 mg/g，비미경처리조건하최대치분별제고료38.3%、39.5%、5.8%。차결과표명，이용저pH처리능구제고양주효모2-10515생산곡광감태능력。
According to shaking flask fermentation curve of saccharomyces cerevisiae 2-10515 for glutathione production, a low pH environment was designed in this study. The seed was trained 6 h under the condition of pH3.5, then can adapt to the long fermentation process of low pH environment so as to promote its ability to synthesize glutathione. The maximum of biomass, intracellular GSH production and intracellular GSH content were 9.7 g/L, 25.6 mg/L, 4.1 mg/g, increased by 38.3 %, 39.5 %, 5.8 %respectively than untreated conditions. The results showed that the GSH production capacity of Saccharomyces cerevisiae 2-10515 was enhanced under the low pH treatment.