食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
21期
103-105
,共3页
白藜芦醇%HPLC%含量测定
白藜蘆醇%HPLC%含量測定
백려호순%HPLC%함량측정
resveratrol%HPLC%determination of content
建立了HPLC测定葡萄酒中白藜芦醇含量的方法，采用色谱柱：THERMO C18（4.6×25 mm，5μm），检测波长：30 nm，流量：1.0 mL/min，进样量：20μL，流动相水-乙腈为65∶35。结果表明：白藜芦醇在0~5μg/mL范围内呈现良好的线性关系，Y=183298X+15201.8（0~5μg/mL）（R2=0.9996）。平均回收率99.7%，RSD=0.75%。本方法简单，快速、准确、重现性好，可以用作葡萄酒中白藜芦醇的含量测定，为评价葡萄酒的品质提供依据。
建立瞭HPLC測定葡萄酒中白藜蘆醇含量的方法，採用色譜柱：THERMO C18（4.6×25 mm，5μm），檢測波長：30 nm，流量：1.0 mL/min，進樣量：20μL，流動相水-乙腈為65∶35。結果錶明：白藜蘆醇在0~5μg/mL範圍內呈現良好的線性關繫，Y=183298X+15201.8（0~5μg/mL）（R2=0.9996）。平均迴收率99.7%，RSD=0.75%。本方法簡單，快速、準確、重現性好，可以用作葡萄酒中白藜蘆醇的含量測定，為評價葡萄酒的品質提供依據。
건립료HPLC측정포도주중백려호순함량적방법，채용색보주：THERMO C18（4.6×25 mm，5μm），검측파장：30 nm，류량：1.0 mL/min，진양량：20μL，류동상수-을정위65∶35。결과표명：백려호순재0~5μg/mL범위내정현량호적선성관계，Y=183298X+15201.8（0~5μg/mL）（R2=0.9996）。평균회수솔99.7%，RSD=0.75%。본방법간단，쾌속、준학、중현성호，가이용작포도주중백려호순적함량측정，위평개포도주적품질제공의거。
To establish a high performance liquid chromatography (HPLC) method for determination content of Resveratrol in wine,20μL samples were tested on a thermo C18 column using water and acetonitrile (65∶35) as mobile phase was with flow rate of 1.0mL/min at 306 nm.The results showed that Resveratrol concentration had good liner relationship within the range of 0-5μg/mL (Y=183 298X+15 201.8,0-5μg/mL,R2=0.999 6). The average recovery was 99.7%with RSD of 0.75%.The method of detection content of Resveratrol in wine by HPLC was simple, fast, accurate, and precise, which could be used to determine content of Resveratrol in wine.To provide the basis for the evaluation of the quality of the wine.
