中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
22期
58-59
,共2页
反相高效液相色谱法%大黄素%大黄酚%保婴镇惊丸%含量测定
反相高效液相色譜法%大黃素%大黃酚%保嬰鎮驚汍%含量測定
반상고효액상색보법%대황소%대황분%보영진량환%함량측정
RP-HPLC%emodin%chrysophanol%Baoyingzhenjing Pill%content determination
目的：建立测定保婴镇惊丸中大黄素和大黄酚含量的反相高效液相色谱法。方法色谱柱为Zorbax SB－C18柱(250 mm ×4.6 mm，5μm)，流动相为甲醇－0.1%磷酸（85：15），检测波长为254 nm，柱温为35℃。结果大黄素、大黄酚进样量分别在0.0412~0.6180μg （ r=0.999999）和0.0906~1.3590μg（ r=0.999998）范围内与峰面积线性关系良好；平均回收率分别为97.02%和98.12%，RSD分别为1.16%和0.94%（ n=6）。结论该方法简便、快速、准确，适用于保婴镇惊丸中大黄素、大黄酚的含量测定。
目的：建立測定保嬰鎮驚汍中大黃素和大黃酚含量的反相高效液相色譜法。方法色譜柱為Zorbax SB－C18柱(250 mm ×4.6 mm，5μm)，流動相為甲醇－0.1%燐痠（85：15），檢測波長為254 nm，柱溫為35℃。結果大黃素、大黃酚進樣量分彆在0.0412~0.6180μg （ r=0.999999）和0.0906~1.3590μg（ r=0.999998）範圍內與峰麵積線性關繫良好；平均迴收率分彆為97.02%和98.12%，RSD分彆為1.16%和0.94%（ n=6）。結論該方法簡便、快速、準確，適用于保嬰鎮驚汍中大黃素、大黃酚的含量測定。
목적：건립측정보영진량환중대황소화대황분함량적반상고효액상색보법。방법색보주위Zorbax SB－C18주(250 mm ×4.6 mm，5μm)，류동상위갑순－0.1%린산（85：15），검측파장위254 nm，주온위35℃。결과대황소、대황분진양량분별재0.0412~0.6180μg （ r=0.999999）화0.0906~1.3590μg（ r=0.999998）범위내여봉면적선성관계량호；평균회수솔분별위97.02%화98.12%，RSD분별위1.16%화0.94%（ n=6）。결론해방법간편、쾌속、준학，괄용우보영진량환중대황소、대황분적함량측정。
Objective To establish a RP-HPLC method for the content determination of emodin and chrysophanol in Baoyingzhenjing Pill. Methods The analysis was performed on the Zorbax SB-C18 column ( 250 mm × 4. 6 mm,5 μm ) with methanol-0. 1% phosphoric acid(85 : 15) as the mobile phase. Emodin and chrysophanol were detected at 254 nm. The column temperature was 35 ℃. Results The linear range was 0. 041 2-0. 618 0 μg( r=0. 999 999)for emodin sample size and 0. 090 6-1. 359 0 μg( r=0. 999 998)for chrysophanol sample size,respectively. The average recovery rates of emodin and chrysophanol were 97. 02% and 98. 12%,RSD were 1. 16% and 0. 94% ( n=6 ) ,respectively. Conclusion This method is simple,rapid,accurate and suitable for determining the contents of emodin and chrysophanol in Baoyingzhenjin Pill.
