浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2014年
22期
1847-1850
,共4页
耐青霉素肺炎链球菌%蛋白质组学%双向电泳%质谱分析
耐青黴素肺炎鏈毬菌%蛋白質組學%雙嚮電泳%質譜分析
내청매소폐염련구균%단백질조학%쌍향전영%질보분석
PRSP%Proteomic%2- DE%Mass spectrometry
目的：观察耐青霉素肺炎链球菌异常蛋白表达，探讨其蛋白组学差异与耐青霉素的关系。方法采用次抑菌浓度法将肺炎链球菌国际标准菌株诱导为耐青霉素肺炎链球菌株；双向电泳分离肺炎链球菌标准株和诱导耐药株的全菌蛋白质；Image Master 2D Platinum 5.0软件对电泳图谱进行分析，寻找表达差异蛋白点；对差异蛋白点进行质谱分析。结果成功诱导耐青霉素肺炎链球菌，并建立肺炎链球菌标准株和耐药株的全菌蛋白双向电泳图谱。标准株和耐药株分别分离出约320，350个蛋白点，发现10个差异蛋白。与标准株比较，ABC转运器、触发因子、DNA聚合酶Ⅲ、氨基酸ABC转运和SP表面蛋白A表达量上调，分子伴侣、脂蛋白、果糖二磷酸醛缩酶、α-烯醇化酶和假想蛋白表达量下调。结论耐青霉素肺炎链球菌蛋白表达异常导致的蛋白组学改变可为深入探讨其耐药机制提供新的研究方向。
目的：觀察耐青黴素肺炎鏈毬菌異常蛋白錶達，探討其蛋白組學差異與耐青黴素的關繫。方法採用次抑菌濃度法將肺炎鏈毬菌國際標準菌株誘導為耐青黴素肺炎鏈毬菌株；雙嚮電泳分離肺炎鏈毬菌標準株和誘導耐藥株的全菌蛋白質；Image Master 2D Platinum 5.0軟件對電泳圖譜進行分析，尋找錶達差異蛋白點；對差異蛋白點進行質譜分析。結果成功誘導耐青黴素肺炎鏈毬菌，併建立肺炎鏈毬菌標準株和耐藥株的全菌蛋白雙嚮電泳圖譜。標準株和耐藥株分彆分離齣約320，350箇蛋白點，髮現10箇差異蛋白。與標準株比較，ABC轉運器、觸髮因子、DNA聚閤酶Ⅲ、氨基痠ABC轉運和SP錶麵蛋白A錶達量上調，分子伴侶、脂蛋白、果糖二燐痠醛縮酶、α-烯醇化酶和假想蛋白錶達量下調。結論耐青黴素肺炎鏈毬菌蛋白錶達異常導緻的蛋白組學改變可為深入探討其耐藥機製提供新的研究方嚮。
목적：관찰내청매소폐염련구균이상단백표체，탐토기단백조학차이여내청매소적관계。방법채용차억균농도법장폐염련구균국제표준균주유도위내청매소폐염련구균주；쌍향전영분리폐염련구균표준주화유도내약주적전균단백질；Image Master 2D Platinum 5.0연건대전영도보진행분석，심조표체차이단백점；대차이단백점진행질보분석。결과성공유도내청매소폐염련구균，병건립폐염련구균표준주화내약주적전균단백쌍향전영도보。표준주화내약주분별분리출약320，350개단백점，발현10개차이단백。여표준주비교，ABC전운기、촉발인자、DNA취합매Ⅲ、안기산ABC전운화SP표면단백A표체량상조，분자반려、지단백、과당이린산철축매、α-희순화매화가상단백표체량하조。결론내청매소폐염련구균단백표체이상도치적단백조학개변가위심입탐토기내약궤제제공신적연구방향。
Objective To analyze the proteomics of penicil in- resistant streptococcus pneumonia (PRSP). Methods The penicil in- resistant streptococcus pneumonia strain was induced by sub- MIC method. The protein profile of Streptococcus pneumoniae and induced PRSP were examined by two- dimensional electrophoresis;and the electrophoretic patterns were ana-lyzed by using Image Master 2D Platinum 5.0. The differential y expressed proteins were further identified by mass spectrometry. Results PRSP strains were induce successful y. Ten differential y expressed proteins were identified between Streptococcus pneumoniae and induced PRSP. The expression of ABC transporter, trigger factor, DNA Polymerase III, amino acid transport, pneumococcal surface protein A was increase in PRSP;while the expression of chaperonin GroES, lipoprotein, fructose bisphos-phate aldolase,α- enolase decreases, hypothetical protein SpneT_02001699 was decreased. Conclusion The findings of pro-teomics change in penicil in- resistant streptococcus pneumoniae may provide laboratory evidence for further study of drug re-sistance mechanisms.
