CAJ | 학술논문

目的：探讨原髓细胞白血病细胞株HL－60细胞半胱氨酸蛋白酶（ Caspase）－9、Smac／DIABLO、细胞色素C（ Cyt－c）和线粒体凋亡诱导蛋白（ Bid）表达及齐墩果酸（ OA）对HL－60细胞线粒体凋亡信号通路的干预作用。方法细胞分瓶培养，实验设空白对照组和12、24、48 h的OA用药组，收集细胞，提取细胞总蛋白、线粒体蛋白和去线粒体细胞浆蛋白，Western印迹法分别检测Caspase－9、Bid、Smac／DIABLO和Cyt－c的表达。结果OA引起HL－60细胞质中Cyt－c和Smac／DIABLO含量增加，并引起Caspase－9的激活，线粒体Bid蛋白含量增加。结论 OA可以通过线粒体凋亡信号通路诱导HL－60细胞凋亡。
목적：탐토원수세포백혈병세포주HL－60세포반광안산단백매（ Caspase）－9、Smac／DIABLO、세포색소C（ Cyt－c）화선립체조망유도단백（ Bid）표체급제돈과산（ OA）대HL－60세포선립체조망신호통로적간예작용。방법세포분병배양，실험설공백대조조화12、24、48 h적OA용약조，수집세포，제취세포총단백、선립체단백화거선립체세포장단백，Western인적법분별검측Caspase－9、Bid、Smac／DIABLO화Cyt－c적표체。결과OA인기HL－60세포질중Cyt－c화Smac／DIABLO함량증가，병인기Caspase－9적격활，선립체Bid단백함량증가。결론 OA가이통과선립체조망신호통로유도HL－60세포조망。
Objective To study the effect of Oleanolic acid to HL-60 cell through mitochondria pathway .Methods The cells were divided into blank control and OA treatment groups , extracted total protein , the expression of Caspase-9 was assayed by Western blot .HL-60 cells were collected from blank control and OA treatment groups , extracted mitochondria from cytoplasm , the expressions of Smac/DIABLO, Cyt-c, Bid were assayed by Western blot .Results OA increased the expressions of Cyt-c and Smac/DIABLO in HL-60 cells, and activated Caspase-9, induced apoptosis by mitochondrial pathway .Bid protein increased in the mitochondria , participate in OA-induced apoptosis in HL-60 cells.Conclusions OA induce apoptosis of HL-60 cells through mitochondrial pathway .