国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
23期
3244-3245,3248
,共3页
梅毒抗体%梅毒酶联免疫吸附试验%梅毒快速血浆反应素试验%梅毒螺旋体颗粒凝集试验%梅毒螺旋体抗体检测-乳胶法
梅毒抗體%梅毒酶聯免疫吸附試驗%梅毒快速血漿反應素試驗%梅毒螺鏇體顆粒凝集試驗%梅毒螺鏇體抗體檢測-乳膠法
매독항체%매독매련면역흡부시험%매독쾌속혈장반응소시험%매독라선체과립응집시험%매독라선체항체검측-유효법
Treponema Pallidum antibody%Treponema Pallidum,i.e.,Treponema Pallidum enzyme-linked immunosorbent as-say%Treponema Pallidum rapid plasma reagin test%Treponema Pallidum particle agglutination test%Treponema Pallidum anti-body detection
目的:比较目前常用的4种梅毒血清检测方法即梅毒酶联免疫吸附试验(TP-ELISA)、梅毒螺旋体颗粒凝集试验(TPPA)、梅毒快速血浆反应素试验(RPR)及梅毒螺旋体抗体检测-乳胶法(TP-AD)在梅毒检测中的价值。方法对5870例门诊及住院患者的标本,先用 TP-ELISA 进行筛查,对查出的121例阳性标本同时做 TP-AD、TPPA 与 RPR 的检测,对检测结果进行比较分析。结果5870例标本用 TP-ELISA 法检出阳性121例,检出率为2.06%。121例阳性标本同时做 TPPA、RPR 及TP-AD 检测,TPPA 检出阳性119例,符合度为98.34%;RPR 检出阳性49例,符合度为40.41%;TP-AD 检出阳性113例,符合度为93.39%。以 TPPA 的检测结果为标准,经过统计学处理,TPPA 与 TP-AD 之间比较,差异无统计学意义(P >0.05),TPPA与 RPR 之间,比较差异有统计学意义(P <0.01)。结论4种方法各有适用性,TP-ELISA 法特异性好、灵敏度高,操作简单,适用于大批孕、产妇及健康人群的体检。TPPA 特异性好、准确性高,适用于确诊,RPR 适用于梅毒活动期患者的监测与疗效观察。TP-AD 与 ELISA 法比较,敏感性略差,特异性好可用于没有特定仪器时的筛查。
目的:比較目前常用的4種梅毒血清檢測方法即梅毒酶聯免疫吸附試驗(TP-ELISA)、梅毒螺鏇體顆粒凝集試驗(TPPA)、梅毒快速血漿反應素試驗(RPR)及梅毒螺鏇體抗體檢測-乳膠法(TP-AD)在梅毒檢測中的價值。方法對5870例門診及住院患者的標本,先用 TP-ELISA 進行篩查,對查齣的121例暘性標本同時做 TP-AD、TPPA 與 RPR 的檢測,對檢測結果進行比較分析。結果5870例標本用 TP-ELISA 法檢齣暘性121例,檢齣率為2.06%。121例暘性標本同時做 TPPA、RPR 及TP-AD 檢測,TPPA 檢齣暘性119例,符閤度為98.34%;RPR 檢齣暘性49例,符閤度為40.41%;TP-AD 檢齣暘性113例,符閤度為93.39%。以 TPPA 的檢測結果為標準,經過統計學處理,TPPA 與 TP-AD 之間比較,差異無統計學意義(P >0.05),TPPA與 RPR 之間,比較差異有統計學意義(P <0.01)。結論4種方法各有適用性,TP-ELISA 法特異性好、靈敏度高,操作簡單,適用于大批孕、產婦及健康人群的體檢。TPPA 特異性好、準確性高,適用于確診,RPR 適用于梅毒活動期患者的鑑測與療效觀察。TP-AD 與 ELISA 法比較,敏感性略差,特異性好可用于沒有特定儀器時的篩查。
목적:비교목전상용적4충매독혈청검측방법즉매독매련면역흡부시험(TP-ELISA)、매독라선체과립응집시험(TPPA)、매독쾌속혈장반응소시험(RPR)급매독라선체항체검측-유효법(TP-AD)재매독검측중적개치。방법대5870례문진급주원환자적표본,선용 TP-ELISA 진행사사,대사출적121례양성표본동시주 TP-AD、TPPA 여 RPR 적검측,대검측결과진행비교분석。결과5870례표본용 TP-ELISA 법검출양성121례,검출솔위2.06%。121례양성표본동시주 TPPA、RPR 급TP-AD 검측,TPPA 검출양성119례,부합도위98.34%;RPR 검출양성49례,부합도위40.41%;TP-AD 검출양성113례,부합도위93.39%。이 TPPA 적검측결과위표준,경과통계학처리,TPPA 여 TP-AD 지간비교,차이무통계학의의(P >0.05),TPPA여 RPR 지간,비교차이유통계학의의(P <0.01)。결론4충방법각유괄용성,TP-ELISA 법특이성호、령민도고,조작간단,괄용우대비잉、산부급건강인군적체검。TPPA 특이성호、준학성고,괄용우학진,RPR 괄용우매독활동기환자적감측여료효관찰。TP-AD 여 ELISA 법비교,민감성략차,특이성호가용우몰유특정의기시적사사。
Objective To compare the value of four kinds of commonly used serological detection method for detecting Trepone-ma Pallidum,i.e.,Treponema Pallidum enzyme-linked immunosorbent assay(TP-ELISA),Treponema pallidum particle agglutina-tion test(TPPA),Treponema Pallidum rapid plasma reagin test(RPR)and Treponema Pallidum antibody detection(TP-AD,emul-sion method).Methods 5 870 specimens from outpatients and inpatients were screened by TP-ELISA.121 cases of detected posi-tive specimen were simultaneously detected by TP-AD,TPPA and RPR.Then the detection results were performed the comparative analysis.Results Among 5 870 specimens,121 cases were positive by ELISA,the detection rate was 2.06%.Among 121 positive cases,119 cases were positive by TPPA,the conformity degree was 98.34%,49 cases were positive by RPR,the conformity degree was 40.41%,113 cases were positive by TP-AD,the conformity degree was 93.38%.With the TPPA results as the standard,there was no statistically significant difference between TPPA and TP-AD(P >0.05),but there was statistically significant difference be-tween TPPA and RPR(P <0.01).Conclusion The four kinds of method have their applicability.ELISA d has good specificity and high sensitivity,and is simple to operate and suitable for the physical examination of large amount of pregnant women,parturients and normal people.TPPA has good specificity and high accuracy,is suitable for definite diagnosis.RPR is suitable for the monito-ring and the curative effect observation in the patients with the active stage of siphilis.Compared with ELISA,TP-AD has slightly less sensitivity,but good specificity and can be used for screening without specific instrument.