CAJ | 학술논문

目的：了解深圳地区临床慢性 HBV 感染患者的 HBV 基因型和预存耐药变异情况。方法应用 PCR 结合反向点杂交法检测244例临床慢性 HBV 感染血清样本的基因型和预存耐药变异情况。结果244例慢性 HBV 感染症状的患者中，共发现有3种 HBV 基因型，其中 B 型143例，占58.6％；C 型100例，占41％；D 型1例，占0.4％；244例慢性 HBV 感染症状的患者中，有38例检出 HBV 预存耐药变异，预存耐药变异的总检出率为15.57％，其中与拉米夫定耐药相关的为22例（9.01％），与阿德福韦酯耐药相关的为16例（6.56％）。结论深圳地区 HBV 基因型以 B、C 型为主，慢性 HBV 感染者其预存耐药率较高。对慢性 HBV 感染者进行 HBV 基因分型及预存耐药检测，对预测疾病进展及指导个体化治疗具有重要意义。
목적：료해심수지구림상만성 HBV 감염환자적 HBV 기인형화예존내약변이정황。방법응용 PCR 결합반향점잡교법검측244례림상만성 HBV 감염혈청양본적기인형화예존내약변이정황。결과244례만성 HBV 감염증상적환자중，공발현유3충 HBV 기인형，기중 B 형143례，점58.6％；C 형100례，점41％；D 형1례，점0.4％；244례만성 HBV 감염증상적환자중，유38례검출 HBV 예존내약변이，예존내약변이적총검출솔위15.57％，기중여랍미부정내약상관적위22례（9.01％），여아덕복위지내약상관적위16례（6.56％）。결론심수지구 HBV 기인형이 B、C 형위주，만성 HBV 감염자기예존내약솔교고。대만성 HBV 감염자진행 HBV 기인분형급예존내약검측，대예측질병진전급지도개체화치료구유중요의의。
Objective To understand the hepatitis B virus(HBV)genotyping and pre-existing antiviral resistance mutation in the patients with clinical chronic HBV infection in Shenzhen area.Methods Serum samples in 244 cases of chronic HBV infection were detected for HBV genotyping and pre-existing antiviral resistance mutation by using PCR combined with reverse dot hybridization. Results In 244 cases of chronic HBV infection,3 kinds of genotype were found,including 143 cases(58.6%)of genotype B,100 ca-ses(41%)of genotype C and 1 case(0.4%)of genotype D;38 cases(15.57%)of pre-existing antiviral resistance mutation were de-tected out.The total detection rate of pre-existing antiviral resistance mutation was 15.57%,in which 22 cases(9.01%)were related with lamivudine resistance and 16 cases(6.56%)were related with adefovir dipivoxil resistance.Conclusion The genotype B and C are the main genotypes in Shenzhen area,and the incidence rate of pre-existing antiviral resistance mutation in the patients with chronic HBV infection is relatively high.The detection of genotyping and pre-existing antiviral resistance mutation of HBV has the important significance to predict the disease progression and guide the individulized treatment.