CAJ | 학술논문

目的：比较液基细胞学检查和 DNA 定量分析法在女性宫颈病变中的应用及其临床意义。方法对参与对比的879例妇女用宫颈刷取材，进行液基薄层制片，分别进行巴氏染色和 DNA 染色。对巴氏染色片做液基细胞学检查，并对 DNA 染色片进行全自动扫描诊断。结果对液基细胞学检查结果显示病变程度在非典型鳞状上皮细胞（ASCUS）意义不明以上病例和全自动 DNA 倍体分析系统检测见异倍体细胞部分病例，建议进一步做阴道镜检查及宫颈活检。28例妇女做了病理活检。以细胞诊断结果为标准，计算出细胞 DNA 定量分析法在 ASCUS 以上宫颈病变的检出率。结论细胞 DNA 定量分析法与液基细胞学检查组合应用，能明显提高宫颈癌及癌前病变的阳性检出率，对我国女性宫颈癌的防治具有重要的意义。
목적：비교액기세포학검사화 DNA 정량분석법재녀성궁경병변중적응용급기림상의의。방법대삼여대비적879례부녀용궁경쇄취재，진행액기박층제편，분별진행파씨염색화 DNA 염색。대파씨염색편주액기세포학검사，병대 DNA 염색편진행전자동소묘진단。결과대액기세포학검사결과현시병변정도재비전형린상상피세포（ASCUS）의의불명이상병례화전자동 DNA 배체분석계통검측견이배체세포부분병례，건의진일보주음도경검사급궁경활검。28례부녀주료병리활검。이세포진단결과위표준，계산출세포 DNA 정량분석법재 ASCUS 이상궁경병변적검출솔。결론세포 DNA 정량분석법여액기세포학검사조합응용，능명현제고궁경암급암전병변적양성검출솔，대아국녀성궁경암적방치구유중요적의의。
Objective To compare the application and clinical significance of the liquid based cytology examination and the DNA quantitative analysis in female cervical lesions.Methods The cervical cell samples were collected from 879 women participating in the comparison by the cervical brush and performed the the liquid-based thin layer section preparation for conducting Papanicolaou staining and DNA staining respectively.The liquid based cytology examination was performed on the Papanicolaou staining section and the fully automatic scanning diagnosis was performed on the DNA staining section.Results The cases of above atypical squa-mous cells of undetermined significance(ASCUS)detected by the liquid based cytology examination and the partial cases of hetero-ploid cell detected by the fully automated DNA ploidy analysis system were recommended to further perform colposcopy and cervi-cal biopsy.28 women were performed the pathological biopsy.With the cytological examination result as the standard,the detection rate of above ASCUS cervical lesions detected by the cellular DNA quantitative analysis was calculated.Conclusion The combined application of the cellular DNA quantitative analysis method and the liquid based cytology examination can obviously increase the positive detection rate of cervical cancer and precancerous lesion,which has important significance for the prevention and treatment of female cervical cancer in our country.