CAJ | 학술논문

目的：探讨雷公藤红素对荷SHG44胶质瘤裸鼠移植瘤生长及血管生成的影响。方法34只SHG －44胶质瘤裸鼠随机分为空白对照组、顺铂组、4 mg／kg雷公藤红素组、2 mg／kg雷公藤红素组和1 mg／kg雷公藤红素组，每周两次，共给药4周。定期观察肿瘤生长情况测量肿瘤体积，计算抑瘤率。免疫组化染色检测移植瘤组织中的MVD、bFGF、VEGF、VEG‐FR1、VEGFR2的蛋白表达。结果4 mg／kg和2 mg／kg的雷公藤红素能抑制肿瘤生长，下调移植瘤组织中VEGFR1、VEG‐FR2的蛋白表达和降低微血管计数（MVD），4 mg／kg的雷公藤红素还能下调荷瘤裸鼠移植瘤组织中bFGF的蛋白表达。结论雷公藤红素能明显抑制荷SHG44胶质瘤裸鼠的移植瘤生长和血管生成，其机制可能与雷公藤红素下调肿瘤组织中bF‐GF、VEGFR1、VEGFR2的蛋白表达有关。
목적：탐토뢰공등홍소대하SHG44효질류라서이식류생장급혈관생성적영향。방법34지SHG －44효질류라서수궤분위공백대조조、순박조、4 mg／kg뢰공등홍소조、2 mg／kg뢰공등홍소조화1 mg／kg뢰공등홍소조，매주량차，공급약4주。정기관찰종류생장정황측량종류체적，계산억류솔。면역조화염색검측이식류조직중적MVD、bFGF、VEGF、VEG‐FR1、VEGFR2적단백표체。결과4 mg／kg화2 mg／kg적뢰공등홍소능억제종류생장，하조이식류조직중VEGFR1、VEG‐FR2적단백표체화강저미혈관계수（MVD），4 mg／kg적뢰공등홍소환능하조하류라서이식류조직중bFGF적단백표체。결론뢰공등홍소능명현억제하SHG44효질류라서적이식류생장화혈관생성，기궤제가능여뢰공등홍소하조종류조직중bF‐GF、VEGFR1、VEGFR2적단백표체유관。
Objective To investigate the effects of celastrol on the SHG44 glioma angiogenesis and growth of nude mouse bearing tumor.Methods 34 SHG44 glioma of nude mouse were randomly divided into control group ,cisplatin group ,4 mg/kg celastrol group ,2 mg/kg celastrol group and 1 mg/kg celastrol group. All mice were received ip five times weekly for four weeks. The xenografts dimensions were measured and the living states of mice observed. The expressions of microvessel densi‐ty (MVD) ,basic fibroblast growth factor(bFGF) vascular endothelial growth factor(VEGF) ,vascular endothelial growth fac‐tor 1(VEGFR1) ,and vascular endothelial growth factor 2(VEGFR2) of transplantation tumor tissue were detected by immu‐nohistochemistry staining.Results The dose of 4 mg/kg and 2 mg/kg celastrol could inhibit the xenografts growth ,down‐reg‐ulate the expression levels of VEGFR1 and VEGFR2 and decrease MVD. The dose of 4 mg/kg celastrol also could down‐regu‐late the expression level of bFGF.Conclusion Celastrol can inhibit the SHG44 glioma angiogenesis and growth of nude mouse bearing tumor ,which is correlated with down‐regulating the expression levels of VEGFR1 ,VEGFR2 and bFGF.