CAJ | 학술논문

目的：研究miR-30c在前列腺癌中的功能调控作用，获得其调控前列腺癌侵袭及转移的可靠证据，进而揭示其在前列腺癌中可能的调控机制。方法从 miRNA 芯片检测结果中获得前列腺癌相关的差异表达的miR-30c分子，进一步通过miR-30c过表达质粒转染前列腺癌细胞，运用Transwell检测细胞侵袭变化，划痕实验检测细胞转移变化。结果 LNCaP/DU145转染质粒后， miR-30c的表达水平显著高于阴性对照组（LNCaP：倍数＝3.87，P＜0.001；Du145：倍数＝4.32， P＜0.001）。Transwell侵袭实验表明，miR-30c转染组的LNCaP/DU145细胞侵袭的数量显著低于对照组（LNCaP：67vs.120个/视野，P＜0.001；DU145：130vs.220个/视野，P＜0.001）。划痕实验结果发现miR-30c转染后，LNCaP/DU145细胞实验组转移的数量显著低于对照组（LNCaP：241 vs.520个/视野，P＜0.001；DU145：490vs.660个/视野，P＜0.001）。结论miR-30c可抑制前列腺癌细胞的侵袭及转移，这说明miR-30c在前列腺中确实起着抑癌的作用，其可能通过KRAS-MAPK信号通路抑制前列腺癌的侵袭及转移。
목적：연구miR-30c재전렬선암중적공능조공작용，획득기조공전렬선암침습급전이적가고증거，진이게시기재전렬선암중가능적조공궤제。방법종 miRNA 심편검측결과중획득전렬선암상관적차이표체적miR-30c분자，진일보통과miR-30c과표체질립전염전렬선암세포，운용Transwell검측세포침습변화，화흔실험검측세포전이변화。결과 LNCaP/DU145전염질립후， miR-30c적표체수평현저고우음성대조조（LNCaP：배수＝3.87，P＜0.001；Du145：배수＝4.32， P＜0.001）。Transwell침습실험표명，miR-30c전염조적LNCaP/DU145세포침습적수량현저저우대조조（LNCaP：67vs.120개/시야，P＜0.001；DU145：130vs.220개/시야，P＜0.001）。화흔실험결과발현miR-30c전염후，LNCaP/DU145세포실험조전이적수량현저저우대조조（LNCaP：241 vs.520개/시야，P＜0.001；DU145：490vs.660개/시야，P＜0.001）。결론miR-30c가억제전렬선암세포적침습급전이，저설명miR-30c재전렬선중학실기착억암적작용，기가능통과KRAS-MAPK신호통로억제전렬선암적침습급전이。
ObjectiveTo study the function of miR-30c which may regulate prostate cancer progression, and we get the reliable evidence that miR-30c regulates the invasion and metastasis of prostate cancer, reveals the possible regulatory mechanism in prostate cancer.MethodsWe chose miR-30c from the differentially expressed miRNAs through miRNA microarray on the human samples. Further by miR-30c overexpression plasmid transfecting prostate cancer cells, using Transwell test cell invasion changed, wound healing test cells shift change.ResultsThe miR-30c expression levels were measured at 48 hours after transfection by qRT-PCR. Data showed that miR-30c expression level in miR-30c-transfected cells was significantly increased compared that in control cells (LNCaP, fold=3.87, P<0.01; Du145, fold=4.32,P<0.01). The results of Transwell assay clearly revealed that overexpression of miR-30c significantly reduced the migrated miR-30c-transfected LNCaP and DU145 cells compared with control cells (LNCaP: 67vs. 120 cells/field,P<0.01; DU145: 130vs. 220 cells/field,P<0.01). The results of wound healing assay indicated that miR-30c markedly reduced the migration of LNCaP and DU145 cells (LNCaP: 241vs. 520 cells/field,P<0.01; DU145: 490vs. 660 cells/field,P<0.01).ConclusionmiR-30c can inhibit invasion and migration of prostate cancer cells, which proved miR-30c can serve as tumor suppressor, and KRAS-MAPK pathway may be involved.