CAJ | 학술논문

目的：对基因表达谱芯片中筛选的差异表达基因 IL-1β进行验证，分析其表达下调与重症继发性肺结核免疫病理的关系。方法（1）用Affymetrix基因表达谱芯片对重症继发性肺结核（重症）、轻症继发性肺结核（轻症）患者和正常对照者（对照）筛选差异表达基因；（2）用实时荧光定量PCR（RT-PCR）扩增来测定重症、轻症患者和对照IL-1β相对表达量；（3）ELISA法测定IL-1β在重症、轻症患者和对照血浆中的蛋白表达量；（4）用方差分析和非参数检验统计方法判断组间比较的差异，P＜0.05为差异有统计学意义。结果（1）芯片筛选差异基因显示重症肺结核患者IL-1β表达下调13.06倍，参与30个信号通路；（2）RT-PCR结果显示IL-1β在重症和对照组表达下调，P＜0.05；（3）ELISA验证IL-1β在重症及轻症组和重症及对照组均表达下调，P＜0.05，与芯片结果相符。结论重症继发性肺结核患者IL-1β表达下调，IL-1β可能是其肺结构免疫病理损伤发生发展的重要因素之一。
목적：대기인표체보심편중사선적차이표체기인 IL-1β진행험증，분석기표체하조여중증계발성폐결핵면역병리적관계。방법（1）용Affymetrix기인표체보심편대중증계발성폐결핵（중증）、경증계발성폐결핵（경증）환자화정상대조자（대조）사선차이표체기인；（2）용실시형광정량PCR（RT-PCR）확증래측정중증、경증환자화대조IL-1β상대표체량；（3）ELISA법측정IL-1β재중증、경증환자화대조혈장중적단백표체량；（4）용방차분석화비삼수검험통계방법판단조간비교적차이，P＜0.05위차이유통계학의의。결과（1）심편사선차이기인현시중증폐결핵환자IL-1β표체하조13.06배，삼여30개신호통로；（2）RT-PCR결과현시IL-1β재중증화대조조표체하조，P＜0.05；（3）ELISA험증IL-1β재중증급경증조화중증급대조조균표체하조，P＜0.05，여심편결과상부。결론중증계발성폐결핵환자IL-1β표체하조，IL-1β가능시기폐결구면역병리손상발생발전적중요인소지일。
ObjectiveTo investigate the relationship between downregulation of IL-1β and immunopathology of severe secondary TB with the gene chip selection results. Methods Differentially expressed genes were screened with Affymetrix Gene expression chips for severe and mild secondary TB patients, and healthy controls (they were severe group and mild group and healthy controls respectively). The relative transcript level of IL-1β was examined by real time PCR (RT-PCR). The protein level of IL-1βwas detected by ELISA. ANOVA and non-parametric tests were used for statistic analysis among the groups.Results The result of gene chip analysis showed that IL-1β wasreduced 13.06 times in severe group compared with mild group patients. IL-1β was involved in 30 signaling pathways. The result of RT-PCR verified that IL-1β wasreduced in severe group versus healthy controls (P<0.05). The result of ELISA verified that IL-1β wasreduced in severe group versus mild group and in severe group versus healthy controls (P<0.05). Conclusion IL-1β wasreduced in severe secondary TB. It may involve in immunopathology mechanism for lung tissue damages.