CAJ | 학술논문

目的：观察人工建立广州管圆线虫生活史动物模型的效果，为进一步开展广州管圆线虫病研究提供基础资料。方法从人工感染广州管圆线虫的福寿螺中分离出3期幼虫，经灌胃感染SD大鼠。感染后第6周从大鼠粪便中分离、计数广州管圆线虫1期幼虫，并将大鼠每天排出的1期幼虫人工感染健康福寿螺。3周后，用胃蛋白酶消化法，从福寿螺内分离出广州管圆线虫3期幼虫。结果 SD大鼠感染广州管圆线虫3期幼虫后第6周，其粪便中均检出1期幼虫，并成功感染健康福寿螺得到3期幼虫。结论在实验室条件下以每只大鼠50条广州管圆线虫3期幼虫的感染量可成功建立广州管圆线虫生活史动物模型及其实验室种群。
목적：관찰인공건립엄주관원선충생활사동물모형적효과，위진일보개전엄주관원선충병연구제공기출자료。방법종인공감염엄주관원선충적복수라중분리출3기유충，경관위감염SD대서。감염후제6주종대서분편중분리、계수엄주관원선충1기유충，병장대서매천배출적1기유충인공감염건강복수라。3주후，용위단백매소화법，종복수라내분리출엄주관원선충3기유충。결과 SD대서감염엄주관원선충3기유충후제6주，기분편중균검출1기유충，병성공감염건강복수라득도3기유충。결론재실험실조건하이매지대서50조엄주관원선충3기유충적감염량가성공건립엄주관원선충생활사동물모형급기실험실충군。
Objective To observe the effect of artificially construction of the life cycle of Angiostrongylus cantonensis in the laboratory condition so as to provide the basis for the research of angiostrongyliasis. Methods SD rats were infected orally with the third?stage larvae of A. cantonensis collected from infected Pomacea canaliculata. Six weeks after the infection the first?stage larvae were isolated and counted from fresh feces of the rats and then were used to infect P. canaliculata. Three weeks lat?er the snails were dissected for counting the third?staged larvae of A. cantonensis. Results The first?stage larvae were detect?ed in the feces of the rats 6 weeks after the infection and the third?staged larvae were successfully isolated after the infection of P. canaliculata. Conclusion The animal model of the entire life cycle of A. cantonensis is successfully established in the labo?ratory with the infection of 50 larvae per rat.