中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
49期
8020-8025
,共6页
刘霞%蒋嘉烨%陆海英%顾翠英%蔡美琴
劉霞%蔣嘉燁%陸海英%顧翠英%蔡美琴
류하%장가엽%륙해영%고취영%채미금
实验动物%组织工程%黑加仑提取物%力竭运动后24 h%核因子κB%肿瘤坏死因子α%肾脏结构
實驗動物%組織工程%黑加崙提取物%力竭運動後24 h%覈因子κB%腫瘤壞死因子α%腎髒結構
실험동물%조직공정%흑가륜제취물%력갈운동후24 h%핵인자κB%종류배사인자α%신장결구
ribes%exercise%kidney%NF-kappa B%tumor necrosis factor-alpha
背景:急性运动后肾脏组织易出现运动性肾缺血再灌注现象,自由基生成增加,发生炎症反应,导致肾功能异常。目的:观察黑加仑提取物对力竭运动后24 h大鼠肾脏结构、肿瘤坏死因子α和核因子κB表达的影响。方法:30只雄性Wistar健康大鼠随机分为3组,每组10只,黑加仑提取物组给予0.44 g/kg黑加仑提取物灌胃,安静对照组、力竭运动后24 h组灌服同等体积的蒸馏水,连续灌胃6周。力竭运动后24 h组和黑加仑提取物组于末次灌胃后进行无负重游泳运动直至力竭疲劳,于24 h后取材,电镜和光镜观察肾脏组织形态和超微结构变化,免疫组化法检测肿瘤坏死因子α蛋白和核因子κB蛋白表达,RT-PCR检测肿瘤坏死因子α mRNA和核因子κB mRNA的表达。结果与结论:与安静对照组比较,力竭运动后24 h组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白高表达,核因子κB mRNA和肿瘤坏死因子α mRNA表达水平极显著增加(P <0.01);与力竭运动后24 h组比较,黑加仑提取物组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白低表达,核因子κB mRNA表达水平显著性降低(P <0.05);肿瘤坏死因子α mRNA表达水平极显著性下降(P<0.01)。力竭运动后24 h组大鼠肾脏发生明显的形态变化和超微结构损伤,黑加仑提取物组大鼠肾脏结构趋于正常。结果表明黑加仑提取物可修复力竭运动后24 h肾脏组织结构,减少炎症因子的表达,防止肾脏发生炎症性损伤。
揹景:急性運動後腎髒組織易齣現運動性腎缺血再灌註現象,自由基生成增加,髮生炎癥反應,導緻腎功能異常。目的:觀察黑加崙提取物對力竭運動後24 h大鼠腎髒結構、腫瘤壞死因子α和覈因子κB錶達的影響。方法:30隻雄性Wistar健康大鼠隨機分為3組,每組10隻,黑加崙提取物組給予0.44 g/kg黑加崙提取物灌胃,安靜對照組、力竭運動後24 h組灌服同等體積的蒸餾水,連續灌胃6週。力竭運動後24 h組和黑加崙提取物組于末次灌胃後進行無負重遊泳運動直至力竭疲勞,于24 h後取材,電鏡和光鏡觀察腎髒組織形態和超微結構變化,免疫組化法檢測腫瘤壞死因子α蛋白和覈因子κB蛋白錶達,RT-PCR檢測腫瘤壞死因子α mRNA和覈因子κB mRNA的錶達。結果與結論:與安靜對照組比較,力竭運動後24 h組中腎髒覈因子κB蛋白和腫瘤壞死因子α蛋白高錶達,覈因子κB mRNA和腫瘤壞死因子α mRNA錶達水平極顯著增加(P <0.01);與力竭運動後24 h組比較,黑加崙提取物組中腎髒覈因子κB蛋白和腫瘤壞死因子α蛋白低錶達,覈因子κB mRNA錶達水平顯著性降低(P <0.05);腫瘤壞死因子α mRNA錶達水平極顯著性下降(P<0.01)。力竭運動後24 h組大鼠腎髒髮生明顯的形態變化和超微結構損傷,黑加崙提取物組大鼠腎髒結構趨于正常。結果錶明黑加崙提取物可脩複力竭運動後24 h腎髒組織結構,減少炎癥因子的錶達,防止腎髒髮生炎癥性損傷。
배경:급성운동후신장조직역출현운동성신결혈재관주현상,자유기생성증가,발생염증반응,도치신공능이상。목적:관찰흑가륜제취물대력갈운동후24 h대서신장결구、종류배사인자α화핵인자κB표체적영향。방법:30지웅성Wistar건강대서수궤분위3조,매조10지,흑가륜제취물조급여0.44 g/kg흑가륜제취물관위,안정대조조、력갈운동후24 h조관복동등체적적증류수,련속관위6주。력갈운동후24 h조화흑가륜제취물조우말차관위후진행무부중유영운동직지력갈피로,우24 h후취재,전경화광경관찰신장조직형태화초미결구변화,면역조화법검측종류배사인자α단백화핵인자κB단백표체,RT-PCR검측종류배사인자α mRNA화핵인자κB mRNA적표체。결과여결론:여안정대조조비교,력갈운동후24 h조중신장핵인자κB단백화종류배사인자α단백고표체,핵인자κB mRNA화종류배사인자α mRNA표체수평겁현저증가(P <0.01);여력갈운동후24 h조비교,흑가륜제취물조중신장핵인자κB단백화종류배사인자α단백저표체,핵인자κB mRNA표체수평현저성강저(P <0.05);종류배사인자α mRNA표체수평겁현저성하강(P<0.01)。력갈운동후24 h조대서신장발생명현적형태변화화초미결구손상,흑가륜제취물조대서신장결구추우정상。결과표명흑가륜제취물가수복력갈운동후24 h신장조직결구,감소염증인자적표체,방지신장발생염증성손상。
BACKGROUND:The kidney tissues easily affected exercise ischemia reperfusion, increased free radicals and inflammation, resulted in abnormal renal function after acute exercise. OBJECTIVE:To observe the influence of Blackcurrant Extract on the structure of kidney and expression of tumor necrosis factor-α and nuclear factor-κB at 24 hours after exhaustiveexercise. METHODS:A total of 30 male Wistar rats were randomly divided into three groups (n=10). Rats in the Blackcurrant Extract group were intragastricaly administered 0.44 g/kg Blackcurrant Extract. Rats in the quietness control group and 24-hour exhaustive exercise group were intragastricaly given an equal volume of distiled water for 6 consecutive weeks. Rats in the 24-hour exhaustive exercise group and Blackcurrant Extract group received no swimming motion until exhaustion fatigue after final intragastric administration. Twenty-four hours later, samples were obtained. Kidney tissue morphology and ultrastructure were observed by electron microscopy and light microscopy. Protein expression of tumor necrosis factor-α and nuclear factor-κB was detected using immunohistochemistry. Tumor necrosis factor-αmRNA and nuclear factor-κB mRNA expression was detected using RT-PCR. RESULTS AND CONCLUSION:Compared with the quietness control group, tumor necrosis factor-α protein and nuclear factor-κB protein expression in the kidney was higher in the 24-hour exhaustive exercise group, and tumor necrosis factor-α mRNA and nuclear factor-κB mRNA expression was significantly increased (P < 0.01). Compared with the 24-hour exhaustive exercise group, tumor necrosis factor-α protein and nuclear factor-κB protein expression was lower in the Blackcurrant Extract group, and nuclear factor-κB mRNA expression was significantly decreased (P < 0.05); tumor necrosis factor-α mRNA expression was significantly reduced (P < 0.01). Kidney in the 24-hour exhaustive exercise group showed obvious morphological changes and ultrastructural damage. The structure of the kidney in the Blackcurrant Extract group tended to be normal. Results suggested that Blackcurrant Extract can repair the kidney tissue injury, reduce the expression of inflammatory factors, and prevent inflammatory damage in the kidney at 24 hours after exhaustive exercise.