中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
49期
7955-7960
,共6页
安文博%姜劲挺%张伦广%张志勇%郑吉元
安文博%薑勁挺%張倫廣%張誌勇%鄭吉元
안문박%강경정%장륜엄%장지용%정길원
实验动物%组织工程%复方倍他米松注射液%腰椎间盘突出症%脊髓%背根神经节%P物质%降钙素基因相关肽%疼痛机制
實驗動物%組織工程%複方倍他米鬆註射液%腰椎間盤突齣癥%脊髓%揹根神經節%P物質%降鈣素基因相關肽%疼痛機製
실험동물%조직공정%복방배타미송주사액%요추간반돌출증%척수%배근신경절%P물질%강개소기인상관태%동통궤제
betamethasone%intervertebral disk displacement%lumbar vertebrae%substance P%calcitonin gene-related peptide
背景：复方倍他米松注射液治疗椎间盘突出症临床应用广泛，但其具体作用机制尚不明确。目的：探讨局部注射复方倍他米松对自体髓核移植模型大鼠脊髓及背根神经节中P物质和降钙素基因相关肽的影响。方法：36只SD大鼠随机分为4组，即：空白组、模型组、假手术组、西药组，每组9只。模型组和西药组适应性喂养1周后手术制作大鼠自体髓核移植模型。于术后第3，7，12天，模型组和假手术组给予128.25μL生理盐水，西药组给予复方倍他米松注射液13.5μL+2%利多卡因注射液67.5μL。末次给药12 h取L4-6节段脊髓及背根神经节，采用免疫荧光染色方法测定两种组织中P物质和降钙素基因相关肽的含量。结果与结论：各组大鼠脊髓、背根神经节中P物质和降钙素基因相关肽平均荧光强度比较，差异有显著性意义(P <0.01)，进一步两两比较：与空白组、假手术组相比较，模型组大鼠脊髓、背根神经节中P物质和降钙素基因相关肽含量显著性增高(P <0.01)，证明模型复制可靠；与模型组、假手术组相比较，西药组大鼠脊髓、背根神经节中P物质和降钙素基因相关肽含量显著性降低(P <0.01)。结果表明复方倍他米松治疗腰椎间盘突出症的作用机制，可能是通过抑制背根神经节神经元合成和分泌P物质，清除背根神经节中P物质和降钙素基因相关肽，减少其向脊髓传递，从而抑制和缓解疼痛。
揹景：複方倍他米鬆註射液治療椎間盤突齣癥臨床應用廣汎，但其具體作用機製尚不明確。目的：探討跼部註射複方倍他米鬆對自體髓覈移植模型大鼠脊髓及揹根神經節中P物質和降鈣素基因相關肽的影響。方法：36隻SD大鼠隨機分為4組，即：空白組、模型組、假手術組、西藥組，每組9隻。模型組和西藥組適應性餵養1週後手術製作大鼠自體髓覈移植模型。于術後第3，7，12天，模型組和假手術組給予128.25μL生理鹽水，西藥組給予複方倍他米鬆註射液13.5μL+2%利多卡因註射液67.5μL。末次給藥12 h取L4-6節段脊髓及揹根神經節，採用免疫熒光染色方法測定兩種組織中P物質和降鈣素基因相關肽的含量。結果與結論：各組大鼠脊髓、揹根神經節中P物質和降鈣素基因相關肽平均熒光彊度比較，差異有顯著性意義(P <0.01)，進一步兩兩比較：與空白組、假手術組相比較，模型組大鼠脊髓、揹根神經節中P物質和降鈣素基因相關肽含量顯著性增高(P <0.01)，證明模型複製可靠；與模型組、假手術組相比較，西藥組大鼠脊髓、揹根神經節中P物質和降鈣素基因相關肽含量顯著性降低(P <0.01)。結果錶明複方倍他米鬆治療腰椎間盤突齣癥的作用機製，可能是通過抑製揹根神經節神經元閤成和分泌P物質，清除揹根神經節中P物質和降鈣素基因相關肽，減少其嚮脊髓傳遞，從而抑製和緩解疼痛。
배경：복방배타미송주사액치료추간반돌출증림상응용엄범，단기구체작용궤제상불명학。목적：탐토국부주사복방배타미송대자체수핵이식모형대서척수급배근신경절중P물질화강개소기인상관태적영향。방법：36지SD대서수궤분위4조，즉：공백조、모형조、가수술조、서약조，매조9지。모형조화서약조괄응성위양1주후수술제작대서자체수핵이식모형。우술후제3，7，12천，모형조화가수술조급여128.25μL생리염수，서약조급여복방배타미송주사액13.5μL+2%리다잡인주사액67.5μL。말차급약12 h취L4-6절단척수급배근신경절，채용면역형광염색방법측정량충조직중P물질화강개소기인상관태적함량。결과여결론：각조대서척수、배근신경절중P물질화강개소기인상관태평균형광강도비교，차이유현저성의의(P <0.01)，진일보량량비교：여공백조、가수술조상비교，모형조대서척수、배근신경절중P물질화강개소기인상관태함량현저성증고(P <0.01)，증명모형복제가고；여모형조、가수술조상비교，서약조대서척수、배근신경절중P물질화강개소기인상관태함량현저성강저(P <0.01)。결과표명복방배타미송치료요추간반돌출증적작용궤제，가능시통과억제배근신경절신경원합성화분비P물질，청제배근신경절중P물질화강개소기인상관태，감소기향척수전체，종이억제화완해동통。
BACKGROUND: Compound betamethasone injection has been widely used to treat intervertebral disc herniation, but its precise mechanism remains unclear. <br> OBJECTIVE: To explore effects of local injection of compound betamethasone on substance P and calcitonin gene-related peptide in spinal cord and dorsal root ganglia of rat models undergoing autologous transplantation of nucleus pulposus. <br> METHODS: A total of 36 Sprague-Dawley rats were randomly assigned to four groups: blank group, model group, sham surgery group, and western medicine group, with 9 rats in each group. After 1 week of adaptive feeding, rat models of autologous transplantation of nucleus pulposus were established in the model and western medicine groups. At 3, 7 and 12 days after surgery, the rats were given 128.25 μL saline in the model and sham surgery groups. The rats in the western medicine group were administered Betamethason Compound Injection 13.5 μL + 2% Lidocaine Injection 67.5 μL. At 12 hours after final administration, L4-6 segments of the spinal cord and dorsal root ganglion were obtained, and substance P and calcitonin gene-related peptide contents in L4-6 segments of the spinal cord and dorsal root ganglion were determined using immunofluorescence staining. <br> RESULTS AND CONCLUSION: Significant differences in mean fluorescence intensity of substance P and calcitonin gene-related peptide were detected in rat spinal cord and dorsal root ganglion in each group (P < 0.01). Further paired comparison showed that compared with the blank and sham surgery groups, substance P and calcitonin gene-related peptide contents were significantly higher in the spinal cord and dorsal root ganglion in the model group (P < 0.01), which verified that models could be replicated and were reliable. Compared with the model and sham surgery groups, substance P and calcitonin gene-related peptide contents were significantly lower in the spinal cord and dorsal root ganglion of rats in the western medicine group (P < 0.01). Above results confirmed that Compound Betamethasone Injection for treating lumbar intervertebral disc herniation eliminated substance P and calcitonin gene-related peptide in dorsal root ganglion possibly by inhibiting dorsal root ganglion neuron synthesis and secreting substance P and reduced their transmission to the spinal cord, resulting in inhibiting and lessening pain.
