CAJ | 학술논문

目的：开展甲胎蛋白异质体和甲胎蛋白联合检测，以早期发现和诊断肝细胞癌（ HCC），鉴别良恶性肝脏肿瘤。方法检测肝细胞癌（ HCC）30例，慢性肝病78例患者血清中AFP-L3和AFP的含量，血清 AFP-L3分离检测采用亲和微量离心纯化柱法，血清中获取的AFP-L3，AFP采用微粒子化学发光免疫法检测，计算AFP-L3占AFP的比率即AFP-L3％。结果30例HCC患者AFP的值为8．8～1524．1 ng／ml，其中AFP-L3％的阳性率为80％，而78例慢性肝病患者中AFP的值为3．2～580．1 ng／ml，AFP-L3％的阳性率为14．1％。 HCC组AFP异质体的阳性率与慢性肝病组有显著差异（P﹤0．01）。结论联合检测甲胎蛋白异质体和甲胎蛋白对HCC，慢性肝炎和肝硬化的患者鉴别诊断，有着重要的临床价值。
목적：개전갑태단백이질체화갑태단백연합검측，이조기발현화진단간세포암（ HCC），감별량악성간장종류。방법검측간세포암（ HCC）30례，만성간병78례환자혈청중AFP-L3화AFP적함량，혈청 AFP-L3분리검측채용친화미량리심순화주법，혈청중획취적AFP-L3，AFP채용미입자화학발광면역법검측，계산AFP-L3점AFP적비솔즉AFP-L3％。결과30례HCC환자AFP적치위8．8～1524．1 ng／ml，기중AFP-L3％적양성솔위80％，이78례만성간병환자중AFP적치위3．2～580．1 ng／ml，AFP-L3％적양성솔위14．1％。 HCC조AFP이질체적양성솔여만성간병조유현저차이（P﹤0．01）。결론연합검측갑태단백이질체화갑태단백대HCC，만성간염화간경화적환자감별진단，유착중요적림상개치。
Objective To early detect and diagnose hepatocellular carcinoma ( HCC) and to identify benign and malignant tumors of liver with joint detection of AFP variant and AFP .Methods AFP-L3 and AFP content in serum of 30 cases of HCC and 78 cases of chronic liver disease were detected .Affinity micro centrifugal purification column was used to separate and de-termine serum AFP-13.Level of AFP-L3 and AFP extracted from the serum was detected by a chemiluminescence immunoassay method using microparticle .The ratio between AFP-L3 and AFP was calculated as AFP-L3%.Results In the 30 patients of HCC,the level of AFP fluctuated between 8.8 ng/ml and 1524.1 ng/ml,among which the positive rate of AFP-L3 was 80%. Whereas in the 78 patients of chronic liver disease ,the level of AFP fluctuated between 3.2 ng/ml and 580.1 ng/ml,among which the positive rate of AFP-L3 was 14.1%.Conclusion The positive rate of AFP variant in HCC group and chronic liver disease group shows significant difference (P﹤0.01).Joint detection of AFP variant and AFP is very important in differential diagnosis of patients with HCC ,chronic hepatitis and cirrhosis .Therefore, it has important clinical value .