CAJ | 학술논문

目的：探索大鼠听觉发育关键期稳定的气导听觉剥夺动物模型的建立方法。方法：选取出生后14天外耳道刚开放的健康 SD 大鼠20只，随机分为实验组（听觉剥夺组）和对照组，每组10只。实验组于第14天应用 TDT 检测ABR 后采用人用助听器耳膜材料，塑形后待其半凝固，沿外耳道方向堵塞双侧外耳道，医用组织胶水及橡胶膜粘合固定后将动物饲养于专门的 SPF 级动物房内，其环境噪声低于60dB，建立新生大鼠气导听觉剥夺模型；对照组于清洁动物房正常声音环境下饲养。两组动物在实验前及实验后第7天、14天、21天及28天分别检测 ABR，进行客观听力学评估。结果：对照组大鼠实验前平均反应阈为（84.5±4.97）dBSPL，实验后第7天、第14天、第21天、第28天分别为（70.5±3.69）dBSPL、（58.5±5.80）dBSPL、（37.0±4.83）dBSPL、（35.5±3.69）dBSPL，在对照组大鼠发育的不同阶段，其听阈逐渐下降，差异有显著统计学意义（P<0.01）；实验组大鼠实验前平均听阈为（83.0±3.50） dBSPL，试验后第7天、第14天、第21天、第28天分别为（79.0±3.16）dBSPL、（80.0±6.24）dBSPL、（79.0±8.10） dBSPL、（77.5±7.17）dBSPL，实验组 ABR 阈值较对照组增高,直到实验后第21天及第28天时仍较对照组显著增高( P <0.01)，实验组 ABR 阈值随出生天数增长逐渐下降趋势不明显,实验组反应阈差异无统计学意义( P >0.05)。结论：采用人用助听器耳膜材料堵塞新生大鼠双侧外耳道，可以建立稳定的气导听觉剥夺动物模型。
목적：탐색대서은각발육관건기은정적기도은각박탈동물모형적건립방법。방법：선취출생후14천외이도강개방적건강 SD 대서20지，수궤분위실험조（은각박탈조）화대조조，매조10지。실험조우제14천응용 TDT 검측ABR 후채용인용조은기이막재료，소형후대기반응고，연외이도방향도새쌍측외이도，의용조직효수급상효막점합고정후장동물사양우전문적 SPF 급동물방내，기배경조성저우60dB，건립신생대서기도은각박탈모형；대조조우청길동물방정상성음배경하사양。량조동물재실험전급실험후제7천、14천、21천급28천분별검측 ABR，진행객관은역학평고。결과：대조조대서실험전평균반응역위（84.5±4.97）dBSPL，실험후제7천、제14천、제21천、제28천분별위（70.5±3.69）dBSPL、（58.5±5.80）dBSPL、（37.0±4.83）dBSPL、（35.5±3.69）dBSPL，재대조조대서발육적불동계단，기은역축점하강，차이유현저통계학의의（P<0.01）；실험조대서실험전평균은역위（83.0±3.50） dBSPL，시험후제7천、제14천、제21천、제28천분별위（79.0±3.16）dBSPL、（80.0±6.24）dBSPL、（79.0±8.10） dBSPL、（77.5±7.17）dBSPL，실험조 ABR 역치교대조조증고,직도실험후제21천급제28천시잉교대조조현저증고( P <0.01)，실험조 ABR 역치수출생천수증장축점하강추세불명현,실험조반응역차이무통계학의의( P >0.05)。결론：채용인용조은기이막재료도새신생대서쌍측외이도，가이건립은정적기도은각박탈동물모형。
Objective To explore a method for establishing stable auditory deprivation rat model of auditory air conduc-tion in the critical period of development. Methods 20 healthy SD rats(born 14 days just after the opening of the ex-ternal auditory canal) were randomly divided into the experimental group (auditory deprivation group, N=10 ) and the control group(N=10). The external auditory canal of the rats in auditory deprivation group were clogged by the hu-man-used hearing aids eardrum which was mouldinged after the semi-solidified and fixed by medical tissue adhesive glue and rubber membrane, and then the rats were feed in a SPF animal room where the environmental noise was below 60dB. The rats in control group were feed in a clean normal voice room. All the rats were detected ABR and objective audiological assessment before the management and in the 7th, 14th, 21st and 28th day after the management. Results Before the experiment ,the average hearing threshold of the Control group was (84.5 ± 4.97) dBSPL, after the experi-ment , the hearing threshold of the 7th, 14th, 21st, and 28th day were (70.5 ±3.69) dBSPL, (58.5 ±5.80) dBSPL, (37.0 ± 4.83) dBSPL , (35.5 ± 3.69) dBSPL respectively, in the control group, the hearing threshold gradually decreased at dif-ferent stages of the development, the difference was statistically significant (P <0.01).Before the experiment, the average hearing threshold of the experimental group was (83.0 ± 3.50) dBSPL, after the experiment ,the hearing threshold of the 7th, 14th, 21st, and 28th day were (79.0 ± 3.16) dBSPL, (80.0 ± 6.24) dBSPL, (79.0 ± 8.10) dBSPL, (77.5 ± 7.17) dBSPL respectively. The ABR threshold of the experimental group was higher than the control group until the experi-ment after the 21st, and 28th day (P <0. 01), The decrease trend of the ARB threshold in the experiment group was not obvious along with the number of days of birth growth, the difference of the threshold in the experimental group was not statistically significant (P> 0. 05). Conclusion we can establish stable auditory deprivation rat model of auditory air conduction in the critical period of development by using the human-used hearing aids eardrum to clog external auditory canal.