CAJ | 학술논문

目的：探讨转化生长因子（TGF-β1）在结直肠癌患者的表达情况及临床意义。方法采用ELISA方法检测50例结直肠癌患者术前、术后，以及25例健康志愿者的外周血TGF-β1表达水平差异，同时检测了结直肠癌肿瘤组织和癌旁正常大肠组织匀浆上清的TGF-β1含量，分析TGF-β1浓度的变化与临床病理指标的关系。结果结直肠癌患者术前血浆TGF-β1水平明显高于术后组以及健康对照组，差异有统计学意义（P<0.05）；肿瘤组织TGF-β1含量显著高于癌旁正常大肠组织（P<0.05）；在CEA阴性及CEA阳性患者中均可分别观察到类似的浓度改变。外周血TGF-β1水平与肿瘤大小呈正相关，随着肿瘤分期的进展，浓度明显升高。结论 TGF-β1可联合CEA检测，更好的为结直肠癌的临床诊断、评价预后和复发提供参考价值，同时为基于抗TGF-β1的肿瘤免疫治疗提供参考依据。
목적：탐토전화생장인자（TGF-β1）재결직장암환자적표체정황급림상의의。방법채용ELISA방법검측50례결직장암환자술전、술후，이급25례건강지원자적외주혈TGF-β1표체수평차이，동시검측료결직장암종류조직화암방정상대장조직균장상청적TGF-β1함량，분석TGF-β1농도적변화여림상병리지표적관계。결과결직장암환자술전혈장TGF-β1수평명현고우술후조이급건강대조조，차이유통계학의의（P<0.05）；종류조직TGF-β1함량현저고우암방정상대장조직（P<0.05）；재CEA음성급CEA양성환자중균가분별관찰도유사적농도개변。외주혈TGF-β1수평여종류대소정정상관，수착종류분기적진전，농도명현승고。결론 TGF-β1가연합CEA검측，경호적위결직장암적림상진단、평개예후화복발제공삼고개치，동시위기우항TGF-β1적종류면역치료제공삼고의거。
Objective To investigate the role of transforming growth factor β1 (TGF-β1) in patients with colorectal cancer. Methods Fresh peripheral blood were obtained from 50 patients (before surgery and at least one week after surgery) and 25 healthy donors in the morning. Fresh colorectal cancer tissues and the adjacent tissues (at least 5 cm from the tumor site) were obtained from patients undergoing tumor resection. The expression levels of TGF-β1 in the blood and tissue specimens were determined using ELISA. Results The plasma levels of TGF-β1 in patients with colorectal cancer were significantly higher than those in the healthy donors, and decreased after the surgery (P<0.05). The tumor tissues expressed higher levels of TGF-β1 than the adjacent tissues from both CEA-negative and-positive patients. The plasma level of TGF-β1 in the patients were positively correlated with the tumor size and clinical tumor stage (P<0.05). Conclusion TGF-β1 combined with CEA can provide important information for the diagnosis, prognostic assessment and prediction of recurrence in patients with colorectal cancer, and may provide new insights for anti-TGF-β1-based tumor immune therapeutic strategies.