CAJ | 학술논문

目的：探讨趋化因子受体7（CXCR7）在胃癌组织及细胞中的表达及意义。方法收集35例胃癌患者的癌组织和癌旁组织，采用RT-PCR及免疫组化分别检测CXCR7 mRNA和蛋白的表达水平，并结合临床资料进行分析；体外培养5种不同分化程度的胃癌细胞系（HGC-27未分化腺癌；MGC-803低分化粘液腺癌；BGC-823低分化腺癌；SGC-7901中分化腺癌；MKN-28高分化腺癌），采用免疫荧光检测各细胞系中CXCR7的表达水平。结果（1）胃癌组织中CXCR7的mRNA和蛋白表达均明显高于癌旁组织（均为P<0.01）；（2）胃癌组织中CXCR7的表达与患者的性别、年龄、吸烟史、Hp感染、肿瘤部位及病理类型等均无明显关系，但有饮酒史的胃癌患者CXCR7的表达高于无饮酒史的患者（P<0.05）；（3）CXCR7在5种不同胃癌细胞系表达程度不一，与分化程度无明显关系，其在中分化胃腺癌细胞系SGC-7901表达程度最高。结论 CXCR7在胃癌组织中呈高表达，在胃癌细胞系中表达程度不一，推测其可能在胃癌的发生发展中具有重要的作用。
목적：탐토추화인자수체7（CXCR7）재위암조직급세포중적표체급의의。방법수집35례위암환자적암조직화암방조직，채용RT-PCR급면역조화분별검측CXCR7 mRNA화단백적표체수평，병결합림상자료진행분석；체외배양5충불동분화정도적위암세포계（HGC-27미분화선암；MGC-803저분화점액선암；BGC-823저분화선암；SGC-7901중분화선암；MKN-28고분화선암），채용면역형광검측각세포계중CXCR7적표체수평。결과（1）위암조직중CXCR7적mRNA화단백표체균명현고우암방조직（균위P<0.01）；（2）위암조직중CXCR7적표체여환자적성별、년령、흡연사、Hp감염、종류부위급병리류형등균무명현관계，단유음주사적위암환자CXCR7적표체고우무음주사적환자（P<0.05）；（3）CXCR7재5충불동위암세포계표체정도불일，여분화정도무명현관계，기재중분화위선암세포계SGC-7901표체정도최고。결론 CXCR7재위암조직중정고표체，재위암세포계중표체정도불일，추측기가능재위암적발생발전중구유중요적작용。
Objective To investigate the expression status of CXCR7 in gastric cancer tissues and cell lines. Methods The expression status of CXCR7 was detected in 35 primary gastric cancer tissues and matched adjacent tissues by immunohistochemistry and RT-PCR. The correlation of CXCR7 expression with the clinicopathological parameters and risk factors of gastric cancer was analyzed. The expression of CXCR7 in gastric cell lines (HGC-27, MGC-803, BGC-823, SGC-7901 and MKN-28) was also detected by immunofluorescence assay. Results The expression of CXCR7 was significantly higher in gastric cancer tissues than in adjacent tissues (P<0.01). CXCR7 expression was not correlated with age, gender, smoking history, Helicobacter pylori infection, tumor location or the pathological type, but showed a higher expression level in patients with a alcohol-drinking history than in those without (P<0.05). CXCR7 was expressed with variable intensities in the 5 gastric cancer cell lines without correlation with the degrees of cell differentiation;its expression was the highest in SGC-7901 cells, a moderately differentiated human gastric adenocarcinoma cell line. Conclusions CXCR7 is highly expressed in gastric cancer tissues with variable intensities in 5 gastric cancer cell lines, suggesting its important role in gastric cancer progression.