CAJ | 학술논문

目的：探讨鳖甲煎丸抗肝细胞癌转移侵袭的分子机制。方法使用含鳖甲煎丸药物血清培养肝癌细胞HepG2，48 h后采用免疫荧光技术检测Wnt/β-catenin通路信号分子β-catenin蛋白表达水平，双荧光素酶检测法检测β-catenin/TCF4复合物的活性，采用RT-PCR技术检测cyclin D1与MMP-2基因转录水平，Western blot技术检测cyclin D1与MMP-2的蛋白表达水平。结果含鳖甲煎丸药物血清可降低肝癌细胞HepG2细胞核中β-catenin蛋白的表达水平，抑制β-catenin/TCF4复合物的活性，明显下调cyclin D1、MMP-2的基因转录及其蛋白的表达水平。结论鳖甲煎丸可抑制Wnt/β-catenin信号通路，从而抑制肝癌细胞HepG2的生长、粘附和转移。
목적：탐토별갑전환항간세포암전이침습적분자궤제。방법사용함별갑전환약물혈청배양간암세포HepG2，48 h후채용면역형광기술검측Wnt/β-catenin통로신호분자β-catenin단백표체수평，쌍형광소매검측법검측β-catenin/TCF4복합물적활성，채용RT-PCR기술검측cyclin D1여MMP-2기인전록수평，Western blot기술검측cyclin D1여MMP-2적단백표체수평。결과함별갑전환약물혈청가강저간암세포HepG2세포핵중β-catenin단백적표체수평，억제β-catenin/TCF4복합물적활성，명현하조cyclin D1、MMP-2적기인전록급기단백적표체수평。결론별갑전환가억제Wnt/β-catenin신호통로，종이억제간암세포HepG2적생장、점부화전이。
Objective To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma. Methods HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR. Results Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities ofβ-catenin/TCF4 complex. Conclusion Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.