南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2014年
12期
1707-1712
,共6页
杏仁中央核%μ-阿片受体%NMDA受体%摄食%摄水
杏仁中央覈%μ-阿片受體%NMDA受體%攝食%攝水
행인중앙핵%μ-아편수체%NMDA수체%섭식%섭수
central nucleus of the amygdala%μ-opioid receptors%NMDA receptors%food intake%water intake
目的：探讨杏仁中央核内的μ-阿片受体对大鼠摄食和摄水的影响，并评估杏仁中央核内的谷氨酸信号系统在阿片肽介导的摄食行为过程中的作用。方法将不锈钢套管植入成年雄性SD大鼠一侧的杏仁中央核内，向饱食或脱水后大鼠杏仁中央核内注射不同剂量的μ-阿片受体选择性激动剂[D-Ala2, N-Me-Phe4, Gly5-ol]-脑啡肽（DAMGO）。注射后60、120和240 min时测量大鼠的摄食量和摄水量。向饱食后大鼠杏仁中央核内联合注射阿片受体非选择性拮抗剂纳曲酮（naloxone, NTX）和DAMGO，或联合注射N-甲基-D-天冬氨酸（NMDA）型谷氨酸受体选择性拮抗剂D-AP-5和DAMGO，注射后60、120和240 min时测量大鼠的摄食量。结果杏仁中央核内注射DAMGO (1~4 nmol)后，饱食大鼠的摄食量明显增加，而脱水大鼠的摄水量变化不大。NTX（26.5 nmol）可拮抗DAMGO诱导的摄食效应，D-AP-5（6.3~25.4 nmol）则对DAMGO诱导的摄食效应没有影响。结论杏仁中央核μ-阿片受体参与对大鼠摄食而不是摄水的调节，这种促食欲作用不依赖于杏仁中央核内的NMDA受体的激活。
目的：探討杏仁中央覈內的μ-阿片受體對大鼠攝食和攝水的影響，併評估杏仁中央覈內的穀氨痠信號繫統在阿片肽介導的攝食行為過程中的作用。方法將不鏽鋼套管植入成年雄性SD大鼠一側的杏仁中央覈內，嚮飽食或脫水後大鼠杏仁中央覈內註射不同劑量的μ-阿片受體選擇性激動劑[D-Ala2, N-Me-Phe4, Gly5-ol]-腦啡肽（DAMGO）。註射後60、120和240 min時測量大鼠的攝食量和攝水量。嚮飽食後大鼠杏仁中央覈內聯閤註射阿片受體非選擇性拮抗劑納麯酮（naloxone, NTX）和DAMGO，或聯閤註射N-甲基-D-天鼕氨痠（NMDA）型穀氨痠受體選擇性拮抗劑D-AP-5和DAMGO，註射後60、120和240 min時測量大鼠的攝食量。結果杏仁中央覈內註射DAMGO (1~4 nmol)後，飽食大鼠的攝食量明顯增加，而脫水大鼠的攝水量變化不大。NTX（26.5 nmol）可拮抗DAMGO誘導的攝食效應，D-AP-5（6.3~25.4 nmol）則對DAMGO誘導的攝食效應沒有影響。結論杏仁中央覈μ-阿片受體參與對大鼠攝食而不是攝水的調節，這種促食欲作用不依賴于杏仁中央覈內的NMDA受體的激活。
목적：탐토행인중앙핵내적μ-아편수체대대서섭식화섭수적영향，병평고행인중앙핵내적곡안산신호계통재아편태개도적섭식행위과정중적작용。방법장불수강투관식입성년웅성SD대서일측적행인중앙핵내，향포식혹탈수후대서행인중앙핵내주사불동제량적μ-아편수체선택성격동제[D-Ala2, N-Me-Phe4, Gly5-ol]-뇌배태（DAMGO）。주사후60、120화240 min시측량대서적섭식량화섭수량。향포식후대서행인중앙핵내연합주사아편수체비선택성길항제납곡동（naloxone, NTX）화DAMGO，혹연합주사N-갑기-D-천동안산（NMDA）형곡안산수체선택성길항제D-AP-5화DAMGO，주사후60、120화240 min시측량대서적섭식량。결과행인중앙핵내주사DAMGO (1~4 nmol)후，포식대서적섭식량명현증가，이탈수대서적섭수량변화불대。NTX（26.5 nmol）가길항DAMGO유도적섭식효응，D-AP-5（6.3~25.4 nmol）칙대DAMGO유도적섭식효응몰유영향。결론행인중앙핵μ-아편수체삼여대대서섭식이불시섭수적조절，저충촉식욕작용불의뢰우행인중앙핵내적NMDA수체적격활。
Objective To investigate the effect of μ-opioid receptors (μ-ORs) in the central nucleus of the amygdala (CeA) on feeding and drinking behaviors in rats and evaluate the role of glutamate signaling in opioid-mediated ingestive behaviors. Methods Stainless steel cannulas were implanted in the unilateral CeA for microinjection of different doses of the selectiveμ-OR agonist DAMGO in satiated or water-deprived male SD rats. The subsequent food intake or water intake of the rats was measured at 60, 120, and 240 min after the injection. The rats receiving microinjections of naloxone (NTX, a nonselective opioid antagonist) or D-AP-5 (a selective N-methyl-D-aspartic acid-type glutamate receptor antagonist) prior to DAMGO microinjection were tested for food intake at 60, 120, and 240 min after the injections. Results Injections of DAMGO (1-4 nmol in 0.5 μl) into the CeA significantly increased food intake in satiated rats, but did not affect water intake in rats with water deprivation. NTX (26.5 nmol in 0.5μl) injected into the CeA antagonized DAMGO-induced feeding but D-AP-5 (6.3-25.4 nmol in 0.5μl) injections did not produce such an effect. Conclusionμ-ORs in the CeA regulate food intake rather than water intake in rats, and the orexigenic role ofμ-ORs is not dependent on the activation of the NMDA receptors in the CeA.
