CAJ | 학술논문

目的：调查临床分离的10株多重耐药鲍曼不动杆菌（MDR-Ab）对常用的氨基糖苷类、β-内酰胺类、氯霉素、喹诺酮类、四环素、磺胺类和利福平等抗菌药物的耐药情况及其携带β-内酰胺类药物耐药基因的存在状况进行调查。方法10株试验菌均分离自2012年1月至2013年12月该院住院患者痰液标本，排除某一患者重复分离菌株。采用K-B纸片扩散法测定21种抗菌药物的敏感性，并用聚合酶链反应（PCR）检测β-内酰胺酶类、金属β-内酰胺酶类、碳青酶烯类及AmpC酶类等耐药基因。结果该MDR-Ab对21种抗菌药物均为耐药，共检出TEM、OXA-2、ADC、CTX-M-1、CTX-M-2、IMP、OXA-24等耐药基因。结论多重耐药鲍曼不动杆菌可能对目前临床常用的β-内酰胺类药物存在着广泛耐药。
목적：조사림상분리적10주다중내약포만불동간균（MDR-Ab）대상용적안기당감류、β-내선알류、록매소、규낙동류、사배소、광알류화리복평등항균약물적내약정황급기휴대β-내선알류약물내약기인적존재상황진행조사。방법10주시험균균분리자2012년1월지2013년12월해원주원환자담액표본，배제모일환자중복분리균주。채용K-B지편확산법측정21충항균약물적민감성，병용취합매련반응（PCR）검측β-내선알매류、금속β-내선알매류、탄청매희류급AmpC매류등내약기인。결과해MDR-Ab대21충항균약물균위내약，공검출TEM、OXA-2、ADC、CTX-M-1、CTX-M-2、IMP、OXA-24등내약기인。결론다중내약포만불동간균가능대목전림상상용적β-내선알류약물존재착엄범내약。
Objective Survey on drug resistance situation of clinically separated 10 multi-drug resistant acinetobacter baumannii(MDR-Ab) for commonly-used beta lactam class,aminoglycosides,chloromycetin,quinolones,sulfonamides and rifampicin and sulfa,etc and existence condition of resistant gene of carried beta lactam drugs. Methods The 10 test bacteria of sputum specimen separated from the inpatients from Jan. 2012 to Dec. 2013 excluding re-separation from a patient was adopted K-B dif-fusion method to detect sensibility of 21 antimicrobial agents,and beta lactam drugs,metal beta la-ctam enzymes,carbapenms and AmpC enzymes. Results The MDR-Ab was resistant to 21 antibacterial drugs,detecting TEM,OXA-2,ADC,CTX-M-1,CTX-M-2、IMP、OXA-24 and so on. Conclusion Multi-drug resistant acinetobacter baumannii(MDR-Ab) may be extensively resistant to currently-used beta lactam drugs.