山东农业大学学报（自然科学版）
山東農業大學學報（自然科學版）
산동농업대학학보（자연과학판）
JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY(NATURAL SCIENCE)
2014年
5期
778-781,784
,共5页
猪殃殃%双氟磺草胺%ALS活性%保护性酶
豬殃殃%雙氟磺草胺%ALS活性%保護性酶
저앙앙%쌍불광초알%ALS활성%보호성매
Galium aparine L.%Florasulam%ALS activity%protective enzymes
本文通过田间试验和室内分析测定，对敏感性猪殃殃（S生物型）和抗性猪殃殃（R生物型）生理指标变化进行了初步研究。试验结果表明：在田间推荐用量上限4.5 g a.i·hm-2双氟磺草胺处理后，R生物型体内的ALS活力表现为先降低后增高趋势，第7 d时达到峰值，是对照的1.4倍。而S生物型体内ALS活力随着处理时间延长也逐渐降低。通过对保护性酶POD、SOD研究结果表明，R生物型喷药后，体内的POD变化呈增高-降低-增高的趋势，第7 d时达到峰值，比对照高16.5 g-1·FW-1，SOD变化趋势则表现为先降低后增高。S生物型在处理后第4 d，体内的POD活性显著下降，7 d时降到最低，而SOD变化则为随着处理时间延长，SOD活性也逐渐降低。综合分析表明，R生物型在受到双氟磺草胺胁迫后，植株能够进行正常生长，靶标酶ALS仍然保持较高的活性，保护性酶POD、SOD持续稳定是对双氟磺草胺产生抗性的主要生理机制。
本文通過田間試驗和室內分析測定，對敏感性豬殃殃（S生物型）和抗性豬殃殃（R生物型）生理指標變化進行瞭初步研究。試驗結果錶明：在田間推薦用量上限4.5 g a.i·hm-2雙氟磺草胺處理後，R生物型體內的ALS活力錶現為先降低後增高趨勢，第7 d時達到峰值，是對照的1.4倍。而S生物型體內ALS活力隨著處理時間延長也逐漸降低。通過對保護性酶POD、SOD研究結果錶明，R生物型噴藥後，體內的POD變化呈增高-降低-增高的趨勢，第7 d時達到峰值，比對照高16.5 g-1·FW-1，SOD變化趨勢則錶現為先降低後增高。S生物型在處理後第4 d，體內的POD活性顯著下降，7 d時降到最低，而SOD變化則為隨著處理時間延長，SOD活性也逐漸降低。綜閤分析錶明，R生物型在受到雙氟磺草胺脅迫後，植株能夠進行正常生長，靶標酶ALS仍然保持較高的活性，保護性酶POD、SOD持續穩定是對雙氟磺草胺產生抗性的主要生理機製。
본문통과전간시험화실내분석측정，대민감성저앙앙（S생물형）화항성저앙앙（R생물형）생리지표변화진행료초보연구。시험결과표명：재전간추천용량상한4.5 g a.i·hm-2쌍불광초알처리후，R생물형체내적ALS활력표현위선강저후증고추세，제7 d시체도봉치，시대조적1.4배。이S생물형체내ALS활력수착처리시간연장야축점강저。통과대보호성매POD、SOD연구결과표명，R생물형분약후，체내적POD변화정증고-강저-증고적추세，제7 d시체도봉치，비대조고16.5 g-1·FW-1，SOD변화추세칙표현위선강저후증고。S생물형재처리후제4 d，체내적POD활성현저하강，7 d시강도최저，이SOD변화칙위수착처리시간연장，SOD활성야축점강저。종합분석표명，R생물형재수도쌍불광초알협박후，식주능구진행정상생장，파표매ALS잉연보지교고적활성，보호성매POD、SOD지속은정시대쌍불광초알산생항성적주요생리궤제。
This paper preliminarily studied the physiological symptom change of sensitive Galium aparine L.(S biotype) and resistant Galium aparine L.(R biotype) through field test and analysis measure in laboratory. The results showed that: the ALS dynamic performance in R biological increased first then decreased after sulfur difluoride alachlor treatment in the field recommended dosage limit 4.5 g a.i hm-2, and reached a peak at 7 d, which is 1.4 times of references. While in S biological, the ALS dynamic performance gradually reduce with the prolong of processing time. Results through the study on protective enzyme POD and SOD showed that POD changes in R biotype after spraying was increasing, reducing and increasing and reach a peak at 7 d, which is 16.5 g-1? FW-1 higher than the reference. SOD changing trend was decreasing first then increasing. S biotype at 4 d after processed. POD activity in the body was significantly decreased after treatment and reached a minimum at 7 d. SOD changes prolonged with the prolonging processing time and SOD activity was also decreased. Comprehensive analysis showed that the plants of R bio-type can normally grow after being stressed by double fluoride sulfur alachlor and neuropathy target esterase ALS remained high activity, the sustained and steady protective enzymes POD, SOD was the main physiological mechanism producing anti-sulfonamide to Florasulam.
