山东农业大学学报（自然科学版）
山東農業大學學報（自然科學版）
산동농업대학학보（자연과학판）
JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY(NATURAL SCIENCE)
2014年
5期
646-650
,共5页
金银花%多糖%复合酶%酶法提取%抗氧化活性
金銀花%多糖%複閤酶%酶法提取%抗氧化活性
금은화%다당%복합매%매법제취%항양화활성
Lonicera japonica%polysaccharide%compound enzymes%enzymatic extraction%antioxidant activities
本文旨在优化金银花多糖的复合酶法提取工艺，并评价金银花多糖的抗氧化活性。以不同复合酶(纤维素酶、果胶酶、木瓜蛋白酶)配比、液料比、pH、酶解温度、酶解时间为试验因素，以金银花多糖得率为考察指标，正交试验筛选复合酶法提取的最佳工艺条件；采用自由基清除能力体系评价金银花多糖的抗氧化活性。结果表明，复合酶最佳配比为纤维素酶2.0%，果胶酶2.0%，木瓜蛋白酶0.5%；复合酶酶解金银花提取多糖的工艺条件为液料比25：1(mL/g)、pH为4.5、酶解温度50℃、酶解时间60 min，在此条件下金银花多糖得率为12.36%；金银花多糖具有较强的抗氧化活性，对DPPH和O2-·自由基的半数抑制浓度分别为0.811 mg/mL、1.363 mg/mL，但与维生素C比较，抗氧化活性较弱。金银花多糖提取工艺方便可行，得率较高，提取到的多糖具有较强的抗氧化活性。
本文旨在優化金銀花多糖的複閤酶法提取工藝，併評價金銀花多糖的抗氧化活性。以不同複閤酶(纖維素酶、果膠酶、木瓜蛋白酶)配比、液料比、pH、酶解溫度、酶解時間為試驗因素，以金銀花多糖得率為攷察指標，正交試驗篩選複閤酶法提取的最佳工藝條件；採用自由基清除能力體繫評價金銀花多糖的抗氧化活性。結果錶明，複閤酶最佳配比為纖維素酶2.0%，果膠酶2.0%，木瓜蛋白酶0.5%；複閤酶酶解金銀花提取多糖的工藝條件為液料比25：1(mL/g)、pH為4.5、酶解溫度50℃、酶解時間60 min，在此條件下金銀花多糖得率為12.36%；金銀花多糖具有較彊的抗氧化活性，對DPPH和O2-·自由基的半數抑製濃度分彆為0.811 mg/mL、1.363 mg/mL，但與維生素C比較，抗氧化活性較弱。金銀花多糖提取工藝方便可行，得率較高，提取到的多糖具有較彊的抗氧化活性。
본문지재우화금은화다당적복합매법제취공예，병평개금은화다당적항양화활성。이불동복합매(섬유소매、과효매、목과단백매)배비、액료비、pH、매해온도、매해시간위시험인소，이금은화다당득솔위고찰지표，정교시험사선복합매법제취적최가공예조건；채용자유기청제능력체계평개금은화다당적항양화활성。결과표명，복합매최가배비위섬유소매2.0%，과효매2.0%，목과단백매0.5%；복합매매해금은화제취다당적공예조건위액료비25：1(mL/g)、pH위4.5、매해온도50℃、매해시간60 min，재차조건하금은화다당득솔위12.36%；금은화다당구유교강적항양화활성，대DPPH화O2-·자유기적반수억제농도분별위0.811 mg/mL、1.363 mg/mL，단여유생소C비교，항양화활성교약。금은화다당제취공예방편가행，득솔교고，제취도적다당구유교강적항양화활성。
In order to optimize the compound enzymatic extraction technology of polysaccharide from Lonicera japonica and and investigate its antioxidant activity. The experiments used polysaccharides extraction rate as response value, and used enzymatic hydrolysi (cellulose, pectinase, papain), the ratio of water to material, pH, extraction temperature and extraction time as experimental factors to optimize the polysaccharide extraction conditions from Lonicera japonica. Antioxidant activity of polysaccharides was measured by DPPH and O2-· free radical elimination method. The best ratio of compound enzyme was 2.0% cellulase, 2.0% pectinase, 0.5% papain. The optimum conditions were as follows, the ratio of water to feedstock was 25 mL/g, pH4.5, extraction temperature was 50℃, extraction time was 60 min. Under the optimal conditions, the experimental extraction rate was 12.36%. IC50 of DPPH and O2-· were 0.811 mg/mL and 1.363 mg/mL, respectively. Antioxidant activity of sample polysaccharides was weaker than those of vitamin C. The optimum compound enzymatic extraction technology of the polysaccharides from Lonicera japonica was convenient and feasible, the polysaccharides extraction rate was higher than other extraction methods, and the extracted polysaccharides had good antioxidant activity.
